ID   RLR28_PLAVT             Reviewed;         243 AA.
AC   A0A172M4N0;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   07-SEP-2016, sequence version 1.
DT   25-MAY-2022, entry version 13.
DE   RecName: Full=Secreted RxLR effector protein 28 {ECO:0000303|Ref.2};
DE   Flags: Precursor;
GN   Name=RxLR28 {ECO:0000303|Ref.2};
OS   Plasmopara viticola (Downy mildew of grapevine) (Botrytis viticola).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Plasmopara.
OX   NCBI_TaxID=143451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=ZJ-1-1;
RX   PubMed=27242731; DOI=10.3389/fmicb.2016.00709;
RA   Xiang J., Li X., Wu J., Yin L., Zhang Y., Lu J.;
RT   "Studying the mechanism of Plasmopara viticola RxLR effectors on
RT   suppressing plant immunity.";
RL   Front. Microbiol. 7:709-709(2016).
RN   [2]
RP   IDENTIFICATION, INDUCTION, AND DOMAIN.
RX   DOI=10.1016/j.pmpp.2015.05.002;
RA   Yin L., Li X., Xiang J., Qu J., Zhang Y., Dry I.B., Lu J.;
RT   "Characterization of the secretome of Plasmopara viticola by de novo
RT   transcriptome analysis.";
RL   Physiol. Mol. Plant Pathol. 91:1-10(2015).
RN   [3]
RP   INDUCTION.
RX   PubMed=30449350; DOI=10.1016/j.funbio.2018.07.006;
RA   Gomez-Zeledon J., Spring O.;
RT   "Up-regulated RxLR effector genes of Plasmopara viticola in synchronized
RT   host-free stages and infected leaves of hosts with different
RT   susceptibility.";
RL   Fungal Biol. 122:1125-1133(2018).
CC   -!- FUNCTION: Effector that significantly enhances susceptibilities of
CC       grapevine and tobacco to pathogens (PubMed:27242731). Acts as a broad
CC       suppressor of cell death to interrupt plant immunity. Completely
CC       inhibits cell death induced by cell death-inducing proteins, including
CC       the PAMP elicitor INF1 from P.infestans (PubMed:27242731). Reduces the
CC       transcriptional levels of the defense-related genes and impairs the
CC       H(2)O(2) accumulation in N.benthamiana (PubMed:27242731).
CC       {ECO:0000269|PubMed:27242731}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27242731}. Host
CC       cytoplasm {ECO:0000269|PubMed:27242731}. Host nucleus
CC       {ECO:0000269|PubMed:27242731}.
CC   -!- INDUCTION: Expression is up-regulated at the earlier infection stages.
CC       {ECO:0000269|PubMed:30449350, ECO:0000269|Ref.2}.
CC   -!- DOMAIN: Has a conserved RxLR motif that acts to carry the protein into
CC       the host cell cytoplasm. Lacks the 'so-called' EER motif, which is
CC       found closely behind the RxLR motif in most of RxLR effector family
CC       members, but the presence of an EER motif is not always essential for
CC       the translocation of every RxLR effector into host cells, or for
CC       inducing a hypersensitive response. {ECO:0000305|Ref.2}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; KX010958; ANC73378.1; -; mRNA.
DR   AlphaFoldDB; A0A172M4N0; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Host cytoplasm; Host nucleus; Secreted; Signal; Virulence.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..243
FT                   /note="Secreted RxLR effector protein 28"
FT                   /id="PRO_5007999411"
FT   REGION          187..243
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           49..52
FT                   /note="RxLR"
FT                   /evidence="ECO:0000305|Ref.2"
FT   COMPBIAS        187..213
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   243 AA;  25471 MW;  0528814FD8B5F71F CRC64;
     MHVSRIIAHI ALATAITATT VSPTDAAWGW TVKSTDEVKA DKRDLVASRG LRGLIGQASP
     NEVSPANVPV SGIPLLGDKQ PSGYPMLPAD SPSTGSSLLN DDLLQENPVP PANGLVHGVP
     LLSSNQPLQT PMLSGNGPIT GYRFFSGNES PMLPANSPIT GLPLLSGNIP STFEGEQISN
     GDEYQQNVDE DKGQNFGHSV SGPPTTTLTG PHTKSGIPPF ENLVAPAKGS MPNTRRNGYQ
     FFE