ID   RLR30_PLAHL             Reviewed;         155 AA.
AC   A0A0P1A8A0;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2016, sequence version 1.
DT   25-MAY-2022, entry version 17.
DE   RecName: Full=Secreted RxLR effector protein RXLR-C301 {ECO:0000303|PubMed:30422341};
DE   Flags: Precursor;
GN   Name=RXLR-C30 {ECO:0000303|PubMed:30422341};
OS   Plasmopara halstedii (Downy mildew of sunflower).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Plasmopara.
OX   NCBI_TaxID=4781;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=26438312; DOI=10.1186/s12864-015-1904-7;
RA   Sharma R., Xia X., Cano L.M., Evangelisti E., Kemen E., Judelson H.,
RA   Oome S., Sambles C., van den Hoogen D.J., Kitner M., Klein J., Meijer H.J.,
RA   Spring O., Win J., Zipper R., Bode H.B., Govers F., Kamoun S.,
RA   Schornack S., Studholme D.J., Van den Ackerveken G., Thines M.;
RT   "Genome analyses of the sunflower pathogen Plasmopara halstedii provide
RT   insights into effector evolution in downy mildews and Phytophthora.";
RL   BMC Genomics 16:741-741(2015).
RN   [2]
RP   DOMAIN, INDUCTION, FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=30422341; DOI=10.1111/tpj.14157;
RA   Pecrix Y., Buendia L., Penouilh-Suzette C., Marechaux M., Legrand L.,
RA   Bouchez O., Rengel D., Gouzy J., Cottret L., Vear F., Godiard L.;
RT   "Sunflower resistance to multiple downy mildew pathotypes revealed by
RT   recognition of conserved effectors of the oomycete Plasmopara halstedii.";
RL   Plant J. 97:730-748(2019).
CC   -!- FUNCTION: Secreted effector that does not suppress pattern-triggered
CC       immunity (PTI) in plant host. {ECO:0000269|PubMed:30422341}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30422341}. Host cell
CC       membrane {ECO:0000269|PubMed:30422341}. Note=Localizes also to the host
CC       tonoplast. {ECO:0000269|PubMed:30422341}.
CC   -!- INDUCTION: Expression is up-regulated during the early plant infection
CC       stages. {ECO:0000269|PubMed:30422341}.
CC   -!- DOMAIN: Has the canonical translocation RxLR motif, but lacks the
CC       canonical EER motif, which characterizes most oomycete effectors
CC       identified so far. {ECO:0000305|PubMed:30422341}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; CCYD01000201; CEG36406.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0P1A8A0; -.
DR   EnsemblProtists; CEG36406; CEG36406; CEG36406.
DR   Proteomes; UP000054928; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Host cell membrane; Host membrane; Membrane; Reference proteome; Secreted;
KW   Signal; Virulence.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..155
FT                   /note="Secreted RxLR effector protein RXLR-C301"
FT                   /id="PRO_5006058451"
FT   MOTIF           34..64
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:30422341"
SQ   SEQUENCE   155 AA;  16978 MW;  63FC6689F3CECCFF CRC64;
     MRLYALSVSL LAAITLLACV IASADSNMVS QMKRRLSQDV SETEITELSE SKKPTAQDID
     NEERAQLTLL SDSQAIVAVA EKAAHSSPKV LGGTSALATT KFALQSQKTS RLRQLLDKVM
     KISGLLKKFL NFFKNKKTNT VPKLENKPHN AFDTV