ID   RLR66_PLAVT             Reviewed;         130 AA.
AC   A0A172M476;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   07-SEP-2016, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Secreted RxLR effector protein 66 {ECO:0000303|PubMed:27242731};
DE   Flags: Precursor;
GN   Name=RxLR66 {ECO:0000303|PubMed:27242731};
OS   Plasmopara viticola (Downy mildew of grapevine) (Botrytis viticola).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Plasmopara.
OX   NCBI_TaxID=143451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, FUNCTION, SUBCELLULAR LOCATION, AND
RP   DOMAIN.
RC   STRAIN=ZJ-1-1;
RX   PubMed=27242731; DOI=10.3389/fmicb.2016.00709;
RA   Xiang J., Li X., Wu J., Yin L., Zhang Y., Lu J.;
RT   "Studying the mechanism of Plasmopara viticola RxLR effectors on
RT   suppressing plant immunity.";
RL   Front. Microbiol. 7:709-709(2016).
CC   -!- FUNCTION: Effector that acts as a broad suppressor of cell death to
CC       interrupt plant immunity. Inhibits cell death induced by cell death-
CC       inducing proteins, including the PAMP elicitor INF1 from P.infestans.
CC       {ECO:0000269|PubMed:27242731}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27242731}. Host
CC       cytoplasm {ECO:0000269|PubMed:27242731}. Host nucleus
CC       {ECO:0000269|PubMed:27242731}. Membrane {ECO:0000255}; Single-pass
CC       membrane protein {ECO:0000255}.
CC   -!- INDUCTION: Expression is up-regulated at later stages of infection.
CC       {ECO:0000269|PubMed:27242731}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:27242731}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; KX010966; ANC73386.1; -; mRNA.
DR   AlphaFoldDB; A0A172M476; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Host cytoplasm; Host nucleus; Membrane; Secreted; Signal; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..130
FT                   /note="Secreted RxLR effector protein 66"
FT                   /id="PRO_5007999407"
FT   TRANSMEM        104..124
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           32..62
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:27242731"
SQ   SEQUENCE   130 AA;  14395 MW;  220A22DF1770B9A9 CRC64;
     MHLRLLMSTV ITATLIVSNN ALTSPSDKTK TRALRGASTV GIAADNLLAA HFSPTLKHKE
     SRGDYNNIQT ERHRKRRLYD APHHPEFYDI AVHQVPSDKS YGGGPAIAIF AGVAATFILI
     DYLIRHFTEN