ID   RLR67_PLAVT             Reviewed;         137 AA.
AC   A0A172M477;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   07-SEP-2016, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Secreted RxLR effector protein 67 {ECO:0000303|PubMed:27242731};
DE   Flags: Precursor;
GN   Name=RxLR67 {ECO:0000303|PubMed:27242731};
OS   Plasmopara viticola (Downy mildew of grapevine) (Botrytis viticola).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Plasmopara.
OX   NCBI_TaxID=143451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, FUNCTION, SUBCELLULAR LOCATION, AND
RP   DOMAIN.
RC   STRAIN=ZJ-1-1;
RX   PubMed=27242731; DOI=10.3389/fmicb.2016.00709;
RA   Xiang J., Li X., Wu J., Yin L., Zhang Y., Lu J.;
RT   "Studying the mechanism of Plasmopara viticola RxLR effectors on
RT   suppressing plant immunity.";
RL   Front. Microbiol. 7:709-709(2016).
RN   [2]
RP   INDUCTION.
RX   PubMed=30449350; DOI=10.1016/j.funbio.2018.07.006;
RA   Gomez-Zeledon J., Spring O.;
RT   "Up-regulated RxLR effector genes of Plasmopara viticola in synchronized
RT   host-free stages and infected leaves of hosts with different
RT   susceptibility.";
RL   Fungal Biol. 122:1125-1133(2018).
CC   -!- FUNCTION: Effector that partially suppresses the tobacco programmed
CC       cell death induced by cell death-inducing proteins.
CC       {ECO:0000269|PubMed:27242731}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27242731}. Host
CC       cytoplasm {ECO:0000269|PubMed:27242731}. Host nucleus
CC       {ECO:0000269|PubMed:27242731}. Membrane {ECO:0000255}; Single-pass
CC       membrane protein {ECO:0000255}.
CC   -!- INDUCTION: Expression is up-regulated at later stages of infection.
CC       {ECO:0000269|PubMed:27242731, ECO:0000269|PubMed:30449350}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:27242731}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; KX010967; ANC73387.1; -; mRNA.
DR   AlphaFoldDB; A0A172M477; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Host cytoplasm; Host nucleus; Membrane; Secreted; Signal; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..137
FT                   /note="Secreted RxLR effector protein 67"
FT                   /id="PRO_5007999425"
FT   TRANSMEM        114..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          40..65
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           32..61
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:27242731"
FT   COMPBIAS        43..58
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   137 AA;  15897 MW;  5FFCA4CCCF6F532D CRC64;
     MRLYILVLAA IAVTLVFASS GPAITYHEVG TRALRQASIT DEKSDDSLNA QAPPLSKSEK
     RLSRSFRTTS RRLPYTNYYH PQYYHPQNYH PHYNYPQYHS SPHVYVHQES KKSWFVRMIL
     EAGIFWAVFH CLSAAFC