ID   RLUA_ECOL6              Reviewed;         219 AA.
AC   Q8FL93;
DT   29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Dual-specificity RNA pseudouridine synthase RluA {ECO:0000250|UniProtKB:P0AA37};
DE            EC=5.4.99.28 {ECO:0000250|UniProtKB:P0AA37};
DE            EC=5.4.99.29 {ECO:0000250|UniProtKB:P0AA37};
DE   AltName: Full=23S rRNA pseudouridine(746) synthase {ECO:0000250|UniProtKB:P0AA37};
DE   AltName: Full=Ribosomal large subunit pseudouridine synthase A {ECO:0000250|UniProtKB:P0AA37};
DE   AltName: Full=rRNA pseudouridylate synthase A {ECO:0000250|UniProtKB:P0AA37};
DE   AltName: Full=rRNA-uridine isomerase A {ECO:0000250|UniProtKB:P0AA37};
DE   AltName: Full=tRNA pseudouridine(32) synthase {ECO:0000250|UniProtKB:P0AA37};
GN   Name=rluA; OrderedLocusNames=c0069;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Dual specificity enzyme that catalyzes the synthesis of
CC       pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32
CC       in the anticodon stem and loop of transfer RNAs.
CC       {ECO:0000250|UniProtKB:P0AA37}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(32) in tRNA = pseudouridine(32) in tRNA;
CC         Xref=Rhea:RHEA:42544, Rhea:RHEA-COMP:10107, Rhea:RHEA-COMP:10108,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.28;
CC         Evidence={ECO:0000250|UniProtKB:P0AA37};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(746) in 23S rRNA = pseudouridine(746) in 23S rRNA;
CC         Xref=Rhea:RHEA:42548, Rhea:RHEA-COMP:10109, Rhea:RHEA-COMP:10110,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.29;
CC         Evidence={ECO:0000250|UniProtKB:P0AA37};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; AE014075; AAN78565.1; -; Genomic_DNA.
DR   RefSeq; WP_000525208.1; NC_004431.1.
DR   AlphaFoldDB; Q8FL93; -.
DR   SMR; Q8FL93; -.
DR   STRING; 199310.c0069; -.
DR   EnsemblBacteria; AAN78565; AAN78565; c0069.
DR   GeneID; 66509862; -.
DR   KEGG; ecc:c0069; -.
DR   eggNOG; COG0564; Bacteria.
DR   HOGENOM; CLU_016902_11_1_6; -.
DR   OMA; HVSQSRM; -.
DR   BioCyc; ECOL199310:C0069-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
PE   3: Inferred from homology;
KW   Isomerase; rRNA processing; tRNA processing.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:P0AA37"
FT   CHAIN           2..219
FT                   /note="Dual-specificity RNA pseudouridine synthase RluA"
FT                   /id="PRO_0000162652"
FT   ACT_SITE        64
FT                   /evidence="ECO:0000250|UniProtKB:P0AA37"
SQ   SEQUENCE   219 AA;  24847 MW;  2E8F20B57EA51D19 CRC64;
     MGMENYNPPQ EPWLVVLYQD DHIMVVNKPS GLLSVPGRLE EHKDSVMTRI QRDYPQAESV
     HRLDMATSGV IVVALTKAAE RELKRQFRER EPKKQYVARV WGHPSPAEGL VDLPLICDWP
     NRPKQKVCYE TGKPAQTEYE VVEYAADNTA RVVLKPITGR SHQLRVHMLA LGHPILGDRF
     YASPEARAMA PRLLLHAEML TITHPAYGNS MTFKAPADF