RLUA_HAEIN
ID RLUA_HAEIN Reviewed; 219 AA.
AC P44782;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Dual-specificity RNA pseudouridine synthase RluA {ECO:0000250|UniProtKB:P0AA37};
DE EC=5.4.99.28 {ECO:0000250|UniProtKB:P0AA37};
DE EC=5.4.99.29 {ECO:0000250|UniProtKB:P0AA37};
DE AltName: Full=23S rRNA pseudouridine(746) synthase {ECO:0000250|UniProtKB:P0AA37};
DE AltName: Full=Ribosomal large subunit pseudouridine synthase A {ECO:0000250|UniProtKB:P0AA37};
DE AltName: Full=rRNA pseudouridylate synthase A {ECO:0000250|UniProtKB:P0AA37};
DE AltName: Full=rRNA-uridine isomerase A {ECO:0000250|UniProtKB:P0AA37};
DE AltName: Full=tRNA pseudouridine(32) synthase {ECO:0000250|UniProtKB:P0AA37};
GN Name=rluA; OrderedLocusNames=HI_0617;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
CC -!- FUNCTION: Dual specificity enzyme that catalyzes the synthesis of
CC pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32
CC in the anticodon stem and loop of transfer RNAs.
CC {ECO:0000250|UniProtKB:P0AA37}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(32) in tRNA = pseudouridine(32) in tRNA;
CC Xref=Rhea:RHEA:42544, Rhea:RHEA-COMP:10107, Rhea:RHEA-COMP:10108,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.28;
CC Evidence={ECO:0000250|UniProtKB:P0AA37};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(746) in 23S rRNA = pseudouridine(746) in 23S rRNA;
CC Xref=Rhea:RHEA:42548, Rhea:RHEA-COMP:10109, Rhea:RHEA-COMP:10110,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.29;
CC Evidence={ECO:0000250|UniProtKB:P0AA37};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
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DR EMBL; L42023; AAC22276.1; -; Genomic_DNA.
DR PIR; E64155; E64155.
DR RefSeq; NP_438775.1; NC_000907.1.
DR RefSeq; WP_005654471.1; NC_000907.1.
DR AlphaFoldDB; P44782; -.
DR SMR; P44782; -.
DR STRING; 71421.HI_0617; -.
DR EnsemblBacteria; AAC22276; AAC22276; HI_0617.
DR KEGG; hin:HI_0617; -.
DR PATRIC; fig|71421.8.peg.641; -.
DR eggNOG; COG0564; Bacteria.
DR HOGENOM; CLU_016902_11_1_6; -.
DR OMA; FTEPAHR; -.
DR PhylomeDB; P44782; -.
DR BioCyc; HINF71421:G1GJ1-638-MON; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; rRNA processing; tRNA processing.
FT CHAIN 1..219
FT /note="Dual-specificity RNA pseudouridine synthase RluA"
FT /id="PRO_0000162655"
FT ACT_SITE 64
FT /evidence="ECO:0000250|UniProtKB:P0AA37"
SQ SEQUENCE 219 AA; 25138 MW; F9A0FA264D882CAF CRC64;
MALIEYNPPL EPYLDIIYQD NHLCVVNKPS GLLSVPGNQP QYYDSAMSRV KEKFGFCEPA
HRLDMATSGI IVFALSKAAD RELKRQFRER EPKKHYQAIV WGHLENDYGE VNLPMICDWE
NRPRQRLDFV LGKRAVTKFE VLARLPNNST RVKLTPVTGR SHQLRLHMLA LGHPILGDKF
YSHPQAKVMS PRLCLHAEEL TITHPITGET MTFNAKSDF
