ID   RLUA_SALTY              Reviewed;         219 AA.
AC   Q8ZRV9;
DT   29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Dual-specificity RNA pseudouridine synthase RluA {ECO:0000250|UniProtKB:P0AA37};
DE            EC=5.4.99.28 {ECO:0000250|UniProtKB:P0AA37};
DE            EC=5.4.99.29 {ECO:0000250|UniProtKB:P0AA37};
DE   AltName: Full=23S rRNA pseudouridine(746) synthase {ECO:0000250|UniProtKB:P0AA37};
DE   AltName: Full=Ribosomal large subunit pseudouridine synthase A {ECO:0000250|UniProtKB:P0AA37};
DE   AltName: Full=rRNA pseudouridylate synthase A {ECO:0000250|UniProtKB:P0AA37};
DE   AltName: Full=rRNA-uridine isomerase A {ECO:0000250|UniProtKB:P0AA37};
DE   AltName: Full=tRNA pseudouridine(32) synthase {ECO:0000250|UniProtKB:P0AA37};
GN   Name=rluA; OrderedLocusNames=STM0095;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
CC   -!- FUNCTION: Dual specificity enzyme that catalyzes the synthesis of
CC       pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32
CC       in the anticodon stem and loop of transfer RNAs.
CC       {ECO:0000250|UniProtKB:P0AA37}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(32) in tRNA = pseudouridine(32) in tRNA;
CC         Xref=Rhea:RHEA:42544, Rhea:RHEA-COMP:10107, Rhea:RHEA-COMP:10108,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.28;
CC         Evidence={ECO:0000250|UniProtKB:P0AA37};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(746) in 23S rRNA = pseudouridine(746) in 23S rRNA;
CC         Xref=Rhea:RHEA:42548, Rhea:RHEA-COMP:10109, Rhea:RHEA-COMP:10110,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.29;
CC         Evidence={ECO:0000250|UniProtKB:P0AA37};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; AE006468; AAL19059.1; -; Genomic_DNA.
DR   RefSeq; NP_459100.1; NC_003197.2.
DR   RefSeq; WP_000525196.1; NC_003197.2.
DR   AlphaFoldDB; Q8ZRV9; -.
DR   SMR; Q8ZRV9; -.
DR   STRING; 99287.STM0095; -.
DR   PaxDb; Q8ZRV9; -.
DR   EnsemblBacteria; AAL19059; AAL19059; STM0095.
DR   GeneID; 1251613; -.
DR   KEGG; stm:STM0095; -.
DR   PATRIC; fig|99287.12.peg.99; -.
DR   HOGENOM; CLU_016902_11_1_6; -.
DR   OMA; HVSQSRM; -.
DR   PhylomeDB; Q8ZRV9; -.
DR   BioCyc; SENT99287:STM0095-MON; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; rRNA processing; tRNA processing.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:P0AA37"
FT   CHAIN           2..219
FT                   /note="Dual-specificity RNA pseudouridine synthase RluA"
FT                   /id="PRO_0000162658"
FT   ACT_SITE        64
FT                   /evidence="ECO:0000250|UniProtKB:P0AA37"
SQ   SEQUENCE   219 AA;  24798 MW;  23B263AB2BB7B359 CRC64;
     MGMENYNPPQ EPWLVILYQD EHIMVVNKPS GLLSVPGRLE AHKDSIMTRI QRDYPQAESV
     HRLDMATSGV IVVALTKAAE RELKRQFRER EPKKQYVARV WGHPSPAEGL VDLPLICDWP
     NRPKQKVCYE TGKPAQTEYN VVEFAADNTA RVVLKPITGR SHQLRVHMLA LGHPILGDRF
     YASPEALSLA PRLQLHAEML TITHPAYGNS MTFKVPADF