RLUA_SHIFL
ID RLUA_SHIFL Reviewed; 219 AA.
AC P0AA38; P39219; Q83MG7;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Dual-specificity RNA pseudouridine synthase RluA {ECO:0000250|UniProtKB:P0AA37};
DE EC=5.4.99.28 {ECO:0000250|UniProtKB:P0AA37};
DE EC=5.4.99.29 {ECO:0000250|UniProtKB:P0AA37};
DE AltName: Full=23S rRNA pseudouridine(746) synthase {ECO:0000250|UniProtKB:P0AA37};
DE AltName: Full=Ribosomal large subunit pseudouridine synthase A {ECO:0000250|UniProtKB:P0AA37};
DE AltName: Full=rRNA pseudouridylate synthase A {ECO:0000250|UniProtKB:P0AA37};
DE AltName: Full=rRNA-uridine isomerase A {ECO:0000250|UniProtKB:P0AA37};
DE AltName: Full=tRNA pseudouridine(32) synthase {ECO:0000250|UniProtKB:P0AA37};
GN Name=rluA; OrderedLocusNames=SF0053, S0055;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Dual specificity enzyme that catalyzes the synthesis of
CC pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32
CC in the anticodon stem and loop of transfer RNAs.
CC {ECO:0000250|UniProtKB:P0AA37}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(32) in tRNA = pseudouridine(32) in tRNA;
CC Xref=Rhea:RHEA:42544, Rhea:RHEA-COMP:10107, Rhea:RHEA-COMP:10108,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.28;
CC Evidence={ECO:0000250|UniProtKB:P0AA37};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(746) in 23S rRNA = pseudouridine(746) in 23S rRNA;
CC Xref=Rhea:RHEA:42548, Rhea:RHEA-COMP:10109, Rhea:RHEA-COMP:10110,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.29;
CC Evidence={ECO:0000250|UniProtKB:P0AA37};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN41719.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE005674; AAN41719.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AE014073; AAP15599.1; -; Genomic_DNA.
DR RefSeq; NP_706012.1; NC_004337.2.
DR RefSeq; WP_000525176.1; NZ_WPGW01000005.1.
DR AlphaFoldDB; P0AA38; -.
DR SMR; P0AA38; -.
DR STRING; 198214.SF0053; -.
DR EnsemblBacteria; AAN41719; AAN41719; SF0053.
DR EnsemblBacteria; AAP15599; AAP15599; S0055.
DR GeneID; 1024562; -.
DR GeneID; 66671653; -.
DR KEGG; sfl:SF0053; -.
DR KEGG; sfx:S0055; -.
DR PATRIC; fig|198214.7.peg.62; -.
DR HOGENOM; CLU_016902_11_1_6; -.
DR OMA; HVSQSRM; -.
DR OrthoDB; 1714699at2; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; rRNA processing; tRNA processing.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P0AA37"
FT CHAIN 2..219
FT /note="Dual-specificity RNA pseudouridine synthase RluA"
FT /id="PRO_0000162659"
FT ACT_SITE 64
FT /evidence="ECO:0000250|UniProtKB:P0AA37"
SQ SEQUENCE 219 AA; 24861 MW; 2ADCE6D779D8C4AB CRC64;
MGMENYNPPQ EPWLVILYQD DHIMVVNKPS GLLSVPGRLE EHKDSVMTRI QRDYPQAESV
HRLDMATSGV IVVALTKAAE RELKRQFRER EPKKQYVARV WGHPSPAEGL VDLPLICDWP
NRPKQKVCYE TGKPAQTEYE VVEYAADNTA RVVLKPITGR SHQLRVHMLA LGHPILGDRF
YASPEARAMA PRLLLHAEML TITHPAYGNS MTFKAPADF
