ID   RLUA_VIBPA              Reviewed;         250 AA.
AC   Q87LD3;
DT   29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Dual-specificity RNA pseudouridine synthase RluA {ECO:0000250|UniProtKB:P0AA37};
DE            EC=5.4.99.28 {ECO:0000250|UniProtKB:P0AA37};
DE            EC=5.4.99.29 {ECO:0000250|UniProtKB:P0AA37};
DE   AltName: Full=23S rRNA pseudouridine(746) synthase {ECO:0000250|UniProtKB:P0AA37};
DE   AltName: Full=Ribosomal large subunit pseudouridine synthase A {ECO:0000250|UniProtKB:P0AA37};
DE   AltName: Full=rRNA pseudouridylate synthase A {ECO:0000250|UniProtKB:P0AA37};
DE   AltName: Full=rRNA-uridine isomerase A {ECO:0000250|UniProtKB:P0AA37};
DE   AltName: Full=tRNA pseudouridine(32) synthase {ECO:0000250|UniProtKB:P0AA37};
GN   Name=rluA; OrderedLocusNames=VP2679;
OS   Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=223926;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RIMD 2210633;
RX   PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA   Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA   Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA   Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT   "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT   distinct from that of V. cholerae.";
RL   Lancet 361:743-749(2003).
CC   -!- FUNCTION: Dual specificity enzyme that catalyzes the synthesis of
CC       pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32
CC       in the anticodon stem and loop of transfer RNAs.
CC       {ECO:0000250|UniProtKB:P0AA37}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(32) in tRNA = pseudouridine(32) in tRNA;
CC         Xref=Rhea:RHEA:42544, Rhea:RHEA-COMP:10107, Rhea:RHEA-COMP:10108,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.28;
CC         Evidence={ECO:0000250|UniProtKB:P0AA37};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(746) in 23S rRNA = pseudouridine(746) in 23S rRNA;
CC         Xref=Rhea:RHEA:42548, Rhea:RHEA-COMP:10109, Rhea:RHEA-COMP:10110,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.29;
CC         Evidence={ECO:0000250|UniProtKB:P0AA37};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; BA000031; BAC60942.1; -; Genomic_DNA.
DR   RefSeq; NP_799058.1; NC_004603.1.
DR   RefSeq; WP_005478830.1; NC_004603.1.
DR   AlphaFoldDB; Q87LD3; -.
DR   SMR; Q87LD3; -.
DR   STRING; 223926.28807689; -.
DR   EnsemblBacteria; BAC60942; BAC60942; BAC60942.
DR   GeneID; 1190224; -.
DR   KEGG; vpa:VP2679; -.
DR   PATRIC; fig|223926.6.peg.2574; -.
DR   eggNOG; COG0564; Bacteria.
DR   HOGENOM; CLU_016902_11_1_6; -.
DR   OMA; HVSQSRM; -.
DR   Proteomes; UP000002493; Chromosome 1.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; rRNA processing; tRNA processing.
FT   CHAIN           1..250
FT                   /note="Dual-specificity RNA pseudouridine synthase RluA"
FT                   /id="PRO_0000162661"
FT   ACT_SITE        64
FT                   /evidence="ECO:0000250|UniProtKB:P0AA37"
SQ   SEQUENCE   250 AA;  29232 MW;  D3BB3C3326849DE0 CRC64;
     MAMLEYNPPT DPWTDIVFED DHILAVNKPS GLLSVPGRLA EHHDSMWSRL QEEYPDIQVV
     HRLDMSTSGL MVLAKNKRAE SALKKQFQFR LTHKIYYARV WGHVEQEEGE IDLPLICDWP
     NRPLQKVCFE DGKPSKTLFQ VAKREEQEDG TKTTIVRLLP ITGRSHQLRV HMQALGHPIV
     GDEFYATEEA KVFSERLELH ASELSFYHPK SHWLRSIFVP CDFYPEAEEM IFDYFDPERK
     LPDYKTLPRP