RLUA_YERPE
ID RLUA_YERPE Reviewed; 206 AA.
AC Q8ZIK1; Q0WJG8;
DT 29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Dual-specificity RNA pseudouridine synthase RluA {ECO:0000250|UniProtKB:P0AA37};
DE EC=5.4.99.28 {ECO:0000250|UniProtKB:P0AA37};
DE EC=5.4.99.29 {ECO:0000250|UniProtKB:P0AA37};
DE AltName: Full=23S rRNA pseudouridine(746) synthase {ECO:0000250|UniProtKB:P0AA37};
DE AltName: Full=Ribosomal large subunit pseudouridine synthase A {ECO:0000250|UniProtKB:P0AA37};
DE AltName: Full=rRNA pseudouridylate synthase A {ECO:0000250|UniProtKB:P0AA37};
DE AltName: Full=rRNA-uridine isomerase A {ECO:0000250|UniProtKB:P0AA37};
DE AltName: Full=tRNA pseudouridine(32) synthase {ECO:0000250|UniProtKB:P0AA37};
GN Name=rluA; OrderedLocusNames=YPO0497, y3678, YP_3682;
OS Yersinia pestis.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=632;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CO-92 / Biovar Orientalis;
RX PubMed=11586360; DOI=10.1038/35097083;
RA Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA Stevens K., Whitehead S., Barrell B.G.;
RT "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL Nature 413:523-527(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KIM10+ / Biovar Mediaevalis;
RX PubMed=12142430; DOI=10.1128/jb.184.16.4601-4611.2002;
RA Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D.,
RA Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A.,
RA Nilles M.L., Matson J.S., Blattner F.R., Perry R.D.;
RT "Genome sequence of Yersinia pestis KIM.";
RL J. Bacteriol. 184:4601-4611(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=91001 / Biovar Mediaevalis;
RX PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA Wang J., Huang P., Yang R.;
RT "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT avirulent to humans.";
RL DNA Res. 11:179-197(2004).
CC -!- FUNCTION: Dual specificity enzyme that catalyzes the synthesis of
CC pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32
CC in the anticodon stem and loop of transfer RNAs.
CC {ECO:0000250|UniProtKB:P0AA37}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(32) in tRNA = pseudouridine(32) in tRNA;
CC Xref=Rhea:RHEA:42544, Rhea:RHEA-COMP:10107, Rhea:RHEA-COMP:10108,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.28;
CC Evidence={ECO:0000250|UniProtKB:P0AA37};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(746) in 23S rRNA = pseudouridine(746) in 23S rRNA;
CC Xref=Rhea:RHEA:42548, Rhea:RHEA-COMP:10109, Rhea:RHEA-COMP:10110,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.29;
CC Evidence={ECO:0000250|UniProtKB:P0AA37};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
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DR EMBL; AL590842; CAL19177.1; -; Genomic_DNA.
DR EMBL; AE009952; AAM87226.1; -; Genomic_DNA.
DR EMBL; AE017042; AAS63830.1; -; Genomic_DNA.
DR PIR; AG0061; AG0061.
DR RefSeq; WP_002210485.1; NZ_WUCM01000024.1.
DR RefSeq; YP_002345570.1; NC_003143.1.
DR AlphaFoldDB; Q8ZIK1; -.
DR SMR; Q8ZIK1; -.
DR STRING; 214092.YPO0497; -.
DR PaxDb; Q8ZIK1; -.
DR DNASU; 1148625; -.
DR EnsemblBacteria; AAM87226; AAM87226; y3678.
DR EnsemblBacteria; AAS63830; AAS63830; YP_3682.
DR GeneID; 66842941; -.
DR KEGG; ype:YPO0497; -.
DR KEGG; ypk:y3678; -.
DR KEGG; ypm:YP_3682; -.
DR PATRIC; fig|214092.21.peg.747; -.
DR eggNOG; COG0564; Bacteria.
DR HOGENOM; CLU_016902_11_1_6; -.
DR OMA; FTEPAHR; -.
DR Proteomes; UP000000815; Chromosome.
DR Proteomes; UP000001019; Chromosome.
DR Proteomes; UP000002490; Chromosome.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; rRNA processing; tRNA processing.
FT CHAIN 1..206
FT /note="Dual-specificity RNA pseudouridine synthase RluA"
FT /id="PRO_0000162663"
FT ACT_SITE 51
FT /evidence="ECO:0000250|UniProtKB:P0AA37"
SQ SEQUENCE 206 AA; 23283 MW; F4951B5C5383F117 CRC64;
MIVLYQDKHI MVVNKPSGLL SVPGRAPENK DSVMTRVQND FPTAESVHRL DMATSGVIVV
ALTKAAEREL KRQFREREPK KTYIARVWGH LAQDEGLIDL PLICDWPNRP KQKVCYETGK
SAQTEYQVIS RDDDGSTRVK LSPITGRSHQ LRVHMLAMGH PILGDGFYAH PEAKAMASRL
QLHAQQLCIT HPEFGTPMHF KCEAEF
