ID   RLUB_BUCAP              Reviewed;         257 AA.
AC   P42395;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2002, sequence version 2.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase B;
DE            EC=5.4.99.22;
DE   AltName: Full=23S rRNA pseudouridine(2605) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase B;
DE   AltName: Full=rRNA-uridine isomerase B;
GN   Name=rluB; OrderedLocusNames=BUsg_271;
OS   Buchnera aphidicola subsp. Schizaphis graminum (strain Sg).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=198804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8407819; DOI=10.1128/jb.175.20.6426-6432.1993;
RA   Munson M.A., Baumann P.;
RT   "Molecular cloning and nucleotide sequence of a putative trpDC(F)BA operon
RT   in Buchnera aphidicola (endosymbiont of the aphid Schizaphis graminum).";
RL   J. Bacteriol. 175:6426-6432(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sg;
RX   PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2605
CC       in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2605) in 23S rRNA = pseudouridine(2605) in 23S rRNA;
CC         Xref=Rhea:RHEA:42520, Rhea:RHEA-COMP:10095, Rhea:RHEA-COMP:10096,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.22;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
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DR   EMBL; Z19055; CAA79503.1; -; Genomic_DNA.
DR   EMBL; AE013218; AAM67829.1; -; Genomic_DNA.
DR   PIR; S36431; S36431.
DR   RefSeq; WP_011053796.1; NC_004061.1.
DR   AlphaFoldDB; P42395; -.
DR   SMR; P42395; -.
DR   STRING; 198804.BUsg_271; -.
DR   EnsemblBacteria; AAM67829; AAM67829; BUsg_271.
DR   KEGG; bas:BUsg_271; -.
DR   eggNOG; COG1187; Bacteria.
DR   HOGENOM; CLU_024979_1_1_6; -.
DR   OMA; SMEFAPF; -.
DR   OrthoDB; 1037615at2; -.
DR   Proteomes; UP000000416; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; RNA-binding; rRNA processing.
FT   CHAIN           1..257
FT                   /note="Ribosomal large subunit pseudouridine synthase B"
FT                   /id="PRO_0000099981"
FT   DOMAIN          3..63
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        109
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        72
FT                   /note="N -> NN (in Ref. 1; CAA79503)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   257 AA;  30099 MW;  48B84BE473BCE8F5 CRC64;
     MTAKIQKILS DLGYGSRRFI ECMIKCGKIS INGEKAIIGQ YLNKKNPGEI LIDKKKIIVK
     RNKNLPKVLI YNKPIGEVCT RDDFQKRLTV FDKLPKLNLN RWVSVGRLDI NTKGLLLFTN
     DGTLANKLMH PRSQIEREYN IRIFGEMNKN KINILRKGVK IIHGYVSFKE IVPLYDKKEG
     KNKWFKGILC EGKNREIRLM FKSIQCQVNQ LIRVRYGNII LPKNLKEGQW MMLNSIFLKK
     LYNLINFDKE IINKKKN