RLUB_BUCBP
ID RLUB_BUCBP Reviewed; 247 AA.
AC Q89AL1;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase B;
DE EC=5.4.99.22;
DE AltName: Full=23S rRNA pseudouridine(2605) synthase;
DE AltName: Full=rRNA pseudouridylate synthase B;
DE AltName: Full=rRNA-uridine isomerase B;
GN Name=rluB; OrderedLocusNames=bbp_262;
OS Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Buchnera.
OX NCBI_TaxID=224915;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bp;
RX PubMed=12522265; DOI=10.1073/pnas.0235981100;
RA van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F.,
RA Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J.,
RA Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.;
RT "Reductive genome evolution in Buchnera aphidicola.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2605
CC in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(2605) in 23S rRNA = pseudouridine(2605) in 23S rRNA;
CC Xref=Rhea:RHEA:42520, Rhea:RHEA-COMP:10095, Rhea:RHEA-COMP:10096,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.22;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
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DR EMBL; AE016826; AAO26988.1; -; Genomic_DNA.
DR RefSeq; WP_011091389.1; NC_004545.1.
DR AlphaFoldDB; Q89AL1; -.
DR SMR; Q89AL1; -.
DR STRING; 224915.bbp_262; -.
DR EnsemblBacteria; AAO26988; AAO26988; bbp_262.
DR GeneID; 56470804; -.
DR KEGG; bab:bbp_262; -.
DR eggNOG; COG1187; Bacteria.
DR HOGENOM; CLU_024979_1_1_6; -.
DR OMA; SMEFAPF; -.
DR OrthoDB; 1037615at2; -.
DR Proteomes; UP000000601; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR Gene3D; 3.30.70.1560; -; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..247
FT /note="Ribosomal large subunit pseudouridine synthase B"
FT /id="PRO_0000099982"
FT DOMAIN 3..73
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 110
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 247 AA; 28994 MW; BD9C5CDCC6ED2320 CRC64;
MCEKIQKILA RHGYGSRRYI ESLIKSHSVK INDEVVKVGQ RISINVIQKV IINNNNYKPK
LNINKSLKVL LYNKPEGEIC TRKDQRNRRT IFEKLPKLSN ARWINIGRLD LNSSGLLLFT
TDGELAYRLT HPSFNIERIY KVRVFGQFTE NILKQLNVRV KLLDGYANFK SIQFKYGEGK
NKWFNVSLCE GRHRIVRRIW EKLGFQVNKL VRISYAHLTL PKTLFPGTWI TLNKHDIDVL
CKKVNLR
