ID   RLUB_ECO57              Reviewed;         290 AA.
AC   Q8X4Q8;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase B;
DE            EC=5.4.99.22;
DE   AltName: Full=23S rRNA pseudouridine(2605) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase B;
DE   AltName: Full=rRNA-uridine isomerase B;
GN   Name=rluB; OrderedLocusNames=Z2541, ECs1841;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2605
CC       in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2605) in 23S rRNA = pseudouridine(2605) in 23S rRNA;
CC         Xref=Rhea:RHEA:42520, Rhea:RHEA-COMP:10095, Rhea:RHEA-COMP:10096,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.22;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
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DR   EMBL; AE005174; AAG56546.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB35264.1; -; Genomic_DNA.
DR   PIR; A99859; A99859.
DR   PIR; F85760; F85760.
DR   RefSeq; NP_309868.1; NC_002695.1.
DR   RefSeq; WP_001291216.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; Q8X4Q8; -.
DR   SMR; Q8X4Q8; -.
DR   STRING; 155864.EDL933_2423; -.
DR   EnsemblBacteria; AAG56546; AAG56546; Z2541.
DR   EnsemblBacteria; BAB35264; BAB35264; ECs_1841.
DR   GeneID; 66674909; -.
DR   GeneID; 912836; -.
DR   KEGG; ece:Z2541; -.
DR   KEGG; ecs:ECs_1841; -.
DR   PATRIC; fig|386585.9.peg.1939; -.
DR   eggNOG; COG1187; Bacteria.
DR   HOGENOM; CLU_024979_1_1_6; -.
DR   OMA; SMEFAPF; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT   CHAIN           1..290
FT                   /note="Ribosomal large subunit pseudouridine synthase B"
FT                   /id="PRO_0000099985"
FT   DOMAIN          3..75
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   REGION          256..290
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        110
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   290 AA;  32597 MW;  51C8FD55CB557D5B CRC64;
     MSEKLQKVLA RAGHGSRREI ESIIEAGRVS VDGKIAKLGD RVEVTPGLKI RIDGHLISVR
     ESAEQICRVL AYYKPEGELC TRNDPEGRPT VFDRLPKLRG ARWIAVGRLD VNTCGLLLFT
     TDGELANRLM HPSREVEREY AVRVFGQVDD AKLRDLSRGV QLEDGPAAFK TIKFSGGEGI
     NQWYNVTLTE GRNREVRRLW EAVGVQVSRL IRVRYGDIPL PKGLPRGGWT ELDLAQTNYL
     RELVELPPET SSKVAVEKDR RRMKANQIRR AVKRHSQVSG GRRSGGRNNG