ID   RLUB_HAEIN              Reviewed;         357 AA.
AC   P45104;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase B;
DE            EC=5.4.99.22;
DE   AltName: Full=23S rRNA pseudouridine(2605) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase B;
DE   AltName: Full=rRNA-uridine isomerase B;
GN   Name=rluB; OrderedLocusNames=HI_1199;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=10675023;
RX   DOI=10.1002/(sici)1522-2683(20000101)21:2<411::aid-elps411>3.0.co;2-4;
RA   Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C.,
RA   Fountoulakis M.;
RT   "Two-dimensional map of the proteome of Haemophilus influenzae.";
RL   Electrophoresis 21:411-429(2000).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2605
CC       in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2605) in 23S rRNA = pseudouridine(2605) in 23S rRNA;
CC         Xref=Rhea:RHEA:42520, Rhea:RHEA-COMP:10095, Rhea:RHEA-COMP:10096,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.22;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
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DR   EMBL; L42023; AAC22853.1; -; Genomic_DNA.
DR   PIR; A64169; A64169.
DR   RefSeq; NP_439355.1; NC_000907.1.
DR   RefSeq; WP_005694244.1; NC_000907.1.
DR   AlphaFoldDB; P45104; -.
DR   SMR; P45104; -.
DR   STRING; 71421.HI_1199; -.
DR   EnsemblBacteria; AAC22853; AAC22853; HI_1199.
DR   KEGG; hin:HI_1199; -.
DR   PATRIC; fig|71421.8.peg.1251; -.
DR   eggNOG; COG1187; Bacteria.
DR   HOGENOM; CLU_024979_1_1_6; -.
DR   OMA; SMEFAPF; -.
DR   PhylomeDB; P45104; -.
DR   BioCyc; HINF71421:G1GJ1-1230-MON; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT   CHAIN           1..357
FT                   /note="Ribosomal large subunit pseudouridine synthase B"
FT                   /id="PRO_0000099986"
FT   DOMAIN          76..148
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   REGION          1..76
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        13..36
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        37..54
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        183
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   357 AA;  40637 MW;  0A31F0D384CBBE82 CRC64;
     MKPSQKQTQR QPHFSKDSAK KRDFSAKNDR RSVSRTARIE TANTKKSAVN SDNKFLSKPK
     AKPVVRASNQ PKAEGEKLQK VLARAGQGSR REIETMIAAG RVSVEGKIAT LGDRIDVHSG
     VKVRIDGQII NLSHTQKEIC RVLMYYKPEG ELCTRSDPEG RATVFDRLPR LTGSRWIAVG
     RLDINTSGLL LFTTDGELAN RLMHPSREVE REYSVRVFGQ VDDAMLARLR KGVQLEDGLA
     NFKEIKFTGG VGINQWYDVT LMEGRNREVR RLWESQGIQV SRLIRIRYGN IKLMKGLPRG
     GWEEMDLENV NYLRELVGLP AETETKLDVK QASRRPKSGQ IRKAVKRYSE MNKRYKK