ID   RLUB_PASMU              Reviewed;         345 AA.
AC   Q9CMY9;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase B;
DE            EC=5.4.99.22;
DE   AltName: Full=23S rRNA pseudouridine(2605) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase B;
DE   AltName: Full=rRNA-uridine isomerase B;
GN   Name=rluB; OrderedLocusNames=PM0661;
OS   Pasteurella multocida (strain Pm70).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Pasteurella.
OX   NCBI_TaxID=272843;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pm70;
RX   PubMed=11248100; DOI=10.1073/pnas.051634598;
RA   May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.;
RT   "Complete genomic sequence of Pasteurella multocida Pm70.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2605
CC       in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2605) in 23S rRNA = pseudouridine(2605) in 23S rRNA;
CC         Xref=Rhea:RHEA:42520, Rhea:RHEA-COMP:10095, Rhea:RHEA-COMP:10096,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.22;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
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DR   EMBL; AE004439; AAK02745.1; -; Genomic_DNA.
DR   RefSeq; WP_010906779.1; NC_002663.1.
DR   AlphaFoldDB; Q9CMY9; -.
DR   SMR; Q9CMY9; -.
DR   STRING; 747.DR93_1491; -.
DR   EnsemblBacteria; AAK02745; AAK02745; PM0661.
DR   KEGG; pmu:PM0661; -.
DR   PATRIC; fig|272843.6.peg.669; -.
DR   HOGENOM; CLU_024979_1_1_6; -.
DR   OMA; SMEFAPF; -.
DR   Proteomes; UP000000809; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT   CHAIN           1..345
FT                   /note="Ribosomal large subunit pseudouridine synthase B"
FT                   /id="PRO_0000099987"
FT   DOMAIN          64..136
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   REGION          1..60
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        20..55
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        171
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   345 AA;  39235 MW;  4503C11582EC55B0 CRC64;
     MKFTKPNSRQ NKPHVEQKTS RRSTNANAVA SKNVKKVTEA QPKSVKASTH SVKPTVKNPL
     VEGEKLQKVL ARAGQGSRRE IEAMIAENRV SVDGKIATLG DRIDVHAGVK IRIDGHLINL
     LHAQKEVCRV LMYYKPEGEL CTRHDPEGRP TVFDRLPRLT GSRWIAVGRL DINTSGLLLF
     TTDGELANRL MHPSREVERE YSVRVFGQVD EAMLHRLKKG VQLEDGPANF KEIKFAGGVG
     MNQWFDVTLM EGRNREVRRL WESQGVQVSR LIRTRYGNIS LMKSLPRGGW EEMDLTNVNY
     LRELVGLPAE VETKLDVTKP RRRAKTGQIR KAVKRYAELN KRYKK