RLUB_PSEAE
ID RLUB_PSEAE Reviewed; 386 AA.
AC Q9HZ55;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase B;
DE EC=5.4.99.22;
DE AltName: Full=23S rRNA pseudouridine(2605) synthase;
DE AltName: Full=rRNA pseudouridylate synthase B;
DE AltName: Full=rRNA-uridine isomerase B;
GN Name=rluB; OrderedLocusNames=PA3179;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2605
CC in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(2605) in 23S rRNA = pseudouridine(2605) in 23S rRNA;
CC Xref=Rhea:RHEA:42520, Rhea:RHEA-COMP:10095, Rhea:RHEA-COMP:10096,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.22;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
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DR EMBL; AE004091; AAG06567.1; -; Genomic_DNA.
DR PIR; E83247; E83247.
DR RefSeq; NP_251869.1; NC_002516.2.
DR RefSeq; WP_003113439.1; NZ_QZGE01000023.1.
DR AlphaFoldDB; Q9HZ55; -.
DR SMR; Q9HZ55; -.
DR STRING; 287.DR97_4757; -.
DR PaxDb; Q9HZ55; -.
DR PRIDE; Q9HZ55; -.
DR EnsemblBacteria; AAG06567; AAG06567; PA3179.
DR GeneID; 882705; -.
DR KEGG; pae:PA3179; -.
DR PATRIC; fig|208964.12.peg.3323; -.
DR PseudoCAP; PA3179; -.
DR HOGENOM; CLU_024979_1_1_6; -.
DR InParanoid; Q9HZ55; -.
DR OMA; MGSRLEM; -.
DR PhylomeDB; Q9HZ55; -.
DR BioCyc; PAER208964:G1FZ6-3239-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR Gene3D; 3.30.70.1560; -; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..386
FT /note="Ribosomal large subunit pseudouridine synthase B"
FT /id="PRO_0000099988"
FT DOMAIN 14..75
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT REGION 260..386
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 267..307
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 320..359
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 371..386
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 119
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 386 AA; 43725 MW; C47840D7C0E5C0CC CRC64;
MNDTPENPHV PVGEKLQKVL ARLGVGSRRD VEVWIAEGRV NVNGSVASLG QRVDSHDAIT
VDGHLIRREE AAESVRRVLI YNKPEGEVCT RDDPEGRPTI FDRLPRLRTG RWINVGRLDI
NTTGLLLFTT DGELANRLMH PSYEMDREYA VRVRGEVTEE MIERLLNGVM LEDGPAKFSD
IQQAPGGEGF NHWYHCVVME GRNREVRRLW ESQGLVVSRL KRVRFGPVFL TSELTMGRYR
EMDQREIDIL SEEVGLKPVA LPGMTTKARE KAERQQRKQA RPLARSERPE AGRKRAPRRE
DGENAARRAP ASRPARGPQP SAERKGREQG TPVAERPRES NRKPRPSKPR DERPASAPGD
KPAARKPQVK RRPKPAGDGM RPGFRR