RLUB_SALTY
ID RLUB_SALTY Reviewed; 291 AA.
AC Q8ZP51;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase B;
DE EC=5.4.99.22;
DE AltName: Full=23S rRNA pseudouridine(2605) synthase;
DE AltName: Full=rRNA pseudouridylate synthase B;
DE AltName: Full=rRNA-uridine isomerase B;
GN Name=rluB; OrderedLocusNames=STM1719;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2605
CC in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(2605) in 23S rRNA = pseudouridine(2605) in 23S rRNA;
CC Xref=Rhea:RHEA:42520, Rhea:RHEA-COMP:10095, Rhea:RHEA-COMP:10096,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.22;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
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DR EMBL; AE006468; AAL20637.1; -; Genomic_DNA.
DR RefSeq; NP_460678.1; NC_003197.2.
DR RefSeq; WP_001291233.1; NC_003197.2.
DR AlphaFoldDB; Q8ZP51; -.
DR SMR; Q8ZP51; -.
DR STRING; 99287.STM1719; -.
DR PaxDb; Q8ZP51; -.
DR EnsemblBacteria; AAL20637; AAL20637; STM1719.
DR GeneID; 1253238; -.
DR KEGG; stm:STM1719; -.
DR PATRIC; fig|99287.12.peg.1816; -.
DR HOGENOM; CLU_024979_1_1_6; -.
DR OMA; SMEFAPF; -.
DR PhylomeDB; Q8ZP51; -.
DR BioCyc; SENT99287:STM1719-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..291
FT /note="Ribosomal large subunit pseudouridine synthase B"
FT /id="PRO_0000099990"
FT DOMAIN 3..63
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 110
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 291 AA; 32612 MW; 91EEC97F773F6EAF CRC64;
MSEKLQKVLA RAGHGSRREI ESIIEAGRVS VDGKIATLGD RVEVTPGLKI RIDGHLISVK
ESAEQICRVL AYYKPEGELC TRNDPEGRPT VFDRLPKLRG ARWIAVGRLD VNTCGLLLFT
TDGELANRLM HPSREVEREY AVRVFGQVDE SKLRDLSRGV QLEDGPAAFK TIKFSGGEGI
NQWYNVTLTE GRNREVRRLW EAVGVQVSRL IRVRYGDIPL PKGLPRGGWT ELDLAQTNYL
RGLVELPPET SSKVAVEKDR RRMKANQIRR AVKRHSQIAG GRRSGGRNNN G
