RLUB_VIBPA
ID RLUB_VIBPA Reviewed; 312 AA.
AC Q87NB7;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase B;
DE EC=5.4.99.22;
DE AltName: Full=23S rRNA pseudouridine(2605) synthase;
DE AltName: Full=rRNA pseudouridylate synthase B;
DE AltName: Full=rRNA-uridine isomerase B;
GN Name=rluB; OrderedLocusNames=VP1951;
OS Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=223926;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIMD 2210633;
RX PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT distinct from that of V. cholerae.";
RL Lancet 361:743-749(2003).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2605
CC in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(2605) in 23S rRNA = pseudouridine(2605) in 23S rRNA;
CC Xref=Rhea:RHEA:42520, Rhea:RHEA-COMP:10095, Rhea:RHEA-COMP:10096,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.22;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
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DR EMBL; BA000031; BAC60214.1; -; Genomic_DNA.
DR RefSeq; NP_798330.1; NC_004603.1.
DR RefSeq; WP_005465081.1; NC_004603.1.
DR AlphaFoldDB; Q87NB7; -.
DR SMR; Q87NB7; -.
DR STRING; 223926.28806943; -.
DR EnsemblBacteria; BAC60214; BAC60214; BAC60214.
DR GeneID; 1189462; -.
DR KEGG; vpa:VP1951; -.
DR PATRIC; fig|223926.6.peg.1866; -.
DR eggNOG; COG1187; Bacteria.
DR HOGENOM; CLU_024979_1_1_6; -.
DR OMA; SMEFAPF; -.
DR Proteomes; UP000002493; Chromosome 1.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..312
FT /note="Ribosomal large subunit pseudouridine synthase B"
FT /id="PRO_0000099993"
FT DOMAIN 3..74
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT REGION 254..312
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 109
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 312 AA; 35996 MW; 6440A74C58CC2893 CRC64;
MSEKLQKVLA RAGHGSRREI ESLIKSGRVS VNGVVAKLGE RLEDESSVVR IDGHIVSAKV
QEEVICRVLA YYKPEGELCT RHDPEGRRTV FDRLPKIRGS RWISVGRLDA NTSGLLLFTT
DGELANRLMH PSRQVEREYL VRVFGEVTEQ KVRNLVKGVE LEDGLARFED VVYAGGEGMN
HTFYVVINEG RNREVRRLWE SQECTVSRLK RVRYGDIFLD KKLPRGGWME LDLKEVNYLR
ELVELRPEKE TMLDLSKDNT SRKRERARSQ KIRRAVKRHE ERVSTSKGRS NNPARRKPKK
NAGEQGARNK HR
