ID   RLUB_VIBVU              Reviewed;         320 AA.
AC   Q8D8C0;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase B;
DE            EC=5.4.99.22;
DE   AltName: Full=23S rRNA pseudouridine(2605) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase B;
DE   AltName: Full=rRNA-uridine isomerase B;
GN   Name=rluB; OrderedLocusNames=VV1_3060;
OS   Vibrio vulnificus (strain CMCP6).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=216895;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CMCP6;
RA   Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.;
RT   "Complete genome sequence of Vibrio vulnificus CMCP6.";
RL   Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2605
CC       in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2605) in 23S rRNA = pseudouridine(2605) in 23S rRNA;
CC         Xref=Rhea:RHEA:42520, Rhea:RHEA-COMP:10095, Rhea:RHEA-COMP:10096,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.22;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE016795; AAO11384.1; -; Genomic_DNA.
DR   RefSeq; WP_011080863.1; NC_004459.3.
DR   AlphaFoldDB; Q8D8C0; -.
DR   SMR; Q8D8C0; -.
DR   EnsemblBacteria; AAO11384; AAO11384; VV1_3060.
DR   GeneID; 66964521; -.
DR   KEGG; vvu:VV1_3060; -.
DR   HOGENOM; CLU_024979_5_2_6; -.
DR   OMA; SMEFAPF; -.
DR   Proteomes; UP000002275; Chromosome 1.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; RNA-binding; rRNA processing.
FT   CHAIN           1..320
FT                   /note="Ribosomal large subunit pseudouridine synthase B"
FT                   /id="PRO_0000099994"
FT   DOMAIN          3..68
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   REGION          254..320
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        285..320
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        109
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   320 AA;  36835 MW;  7F2F973BD3BBEC26 CRC64;
     MNEKLQKVLA RAGHGSRREL EALIKSGRVS VNGIVAKLGE RLEDEQAVVR IDGHVVSVKA
     QEEVICRVLA YYKPEGELCT RHDPEGRRTV FDRLPKIRGS RWISVGRLDA NTSGLLLFTT
     DGELANRLMH PSRQVEREYL VRVFGEVNEH KVRNLVNGVE LDDGLARFED VVYTGGEGMN
     HTFYVVINEG RNREVRRLWE SQDCTVSRLK RVRYGDIYLD KKLPRGGWME LELKDVNYLR
     ELVELRPEKE TMIELNNSSR KRDRARSQKI RRAVKRHEER VSTPKGRSNN AARRKPSKAD
     GQTNKTNSRN QAGSRSKPQR