RLUB_YERPE
ID RLUB_YERPE Reviewed; 318 AA.
AC Q8ZEG0; Q0WEV4; Q8D0J6;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase B;
DE EC=5.4.99.22;
DE AltName: Full=23S rRNA pseudouridine(2605) synthase;
DE AltName: Full=rRNA pseudouridylate synthase B;
DE AltName: Full=rRNA-uridine isomerase B;
GN Name=rluB; OrderedLocusNames=YPO2213, y2054, YP_2010;
OS Yersinia pestis.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=632;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CO-92 / Biovar Orientalis;
RX PubMed=11586360; DOI=10.1038/35097083;
RA Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA Stevens K., Whitehead S., Barrell B.G.;
RT "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL Nature 413:523-527(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KIM10+ / Biovar Mediaevalis;
RX PubMed=12142430; DOI=10.1128/jb.184.16.4601-4611.2002;
RA Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D.,
RA Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A.,
RA Nilles M.L., Matson J.S., Blattner F.R., Perry R.D.;
RT "Genome sequence of Yersinia pestis KIM.";
RL J. Bacteriol. 184:4601-4611(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=91001 / Biovar Mediaevalis;
RX PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA Wang J., Huang P., Yang R.;
RT "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT avirulent to humans.";
RL DNA Res. 11:179-197(2004).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2605
CC in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(2605) in 23S rRNA = pseudouridine(2605) in 23S rRNA;
CC Xref=Rhea:RHEA:42520, Rhea:RHEA-COMP:10095, Rhea:RHEA-COMP:10096,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.22;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM85618.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAS62226.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AL590842; CAL20842.1; -; Genomic_DNA.
DR EMBL; AE009952; AAM85618.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE017042; AAS62226.1; ALT_INIT; Genomic_DNA.
DR PIR; AG0269; AG0269.
DR AlphaFoldDB; Q8ZEG0; -.
DR SMR; Q8ZEG0; -.
DR STRING; 214092.YPO2213; -.
DR PaxDb; Q8ZEG0; -.
DR DNASU; 1147001; -.
DR EnsemblBacteria; AAM85618; AAM85618; y2054.
DR EnsemblBacteria; AAS62226; AAS62226; YP_2010.
DR KEGG; ype:YPO2213; -.
DR KEGG; ypj:CH55_303; -.
DR KEGG; ypk:y2054; -.
DR KEGG; ypl:CH46_2900; -.
DR KEGG; ypm:YP_2010; -.
DR KEGG; ypv:BZ15_1328; -.
DR KEGG; ypw:CH59_3908; -.
DR PATRIC; fig|632.151.peg.1299; -.
DR eggNOG; COG1187; Bacteria.
DR HOGENOM; CLU_024979_1_1_6; -.
DR OMA; SMEFAPF; -.
DR Proteomes; UP000000815; Chromosome.
DR Proteomes; UP000001019; Chromosome.
DR Proteomes; UP000002490; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..318
FT /note="Ribosomal large subunit pseudouridine synthase B"
FT /id="PRO_0000099999"
FT DOMAIN 3..75
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT REGION 271..318
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 288..305
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 110
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 318 AA; 35695 MW; E51A3A96395C1A4F CRC64;
MGEKLQKILA RAGHGSRREI EAIIQQGRVS VDGKVSKLGD RVEVTQSTKI RLDGHLLSIK
ESEENVCRVL AYYKPEGELC TRNDPEGRPT VFDRLPKLRG SRWVAVGRLD VNTSGLLLFT
TDGELANRLM HPSREVEREY AVRVFGQIDD DKIKQLSRGV QLEDGPAAFR TISYQGGEGI
NQWYNVTLTE GRNREVRRLW EAVGVQVSRL IRVRYGDINL PKGLPRGGWT ELDLKATNYL
RELVELDVET VSKLPVEKDR RRVKANQIRR AVKRHTEVSG RQVAGRQGSA RKGSTRQNVG
NAAPAATASR RSGPKKRG
