RLUC_BUCAI
ID RLUC_BUCAI Reviewed; 314 AA.
AC P57430;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase C;
DE EC=5.4.99.24;
DE AltName: Full=23S rRNA pseudouridine(955/2504/2580) synthase;
DE AltName: Full=rRNA pseudouridylate synthase C;
DE AltName: Full=rRNA-uridine isomerase C;
GN Name=rluC; OrderedLocusNames=BU348;
OS Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS pisum symbiotic bacterium).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Buchnera.
OX NCBI_TaxID=107806;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=APS;
RX PubMed=10993077; DOI=10.1038/35024074;
RA Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT sp. APS.";
RL Nature 407:81-86(2000).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC positions 955, 2504 and 2580 in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(955/2504/2580) in 23S rRNA =
CC pseudouridine(955/2504/2580) in 23S rRNA; Xref=Rhea:RHEA:42528,
CC Rhea:RHEA-COMP:10099, Rhea:RHEA-COMP:10100, ChEBI:CHEBI:65314,
CC ChEBI:CHEBI:65315; EC=5.4.99.24;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
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DR EMBL; BA000003; BAB13053.1; -; Genomic_DNA.
DR RefSeq; NP_240167.1; NC_002528.1.
DR RefSeq; WP_009874303.1; NC_002528.1.
DR AlphaFoldDB; P57430; -.
DR SMR; P57430; -.
DR STRING; 107806.10039019; -.
DR EnsemblBacteria; BAB13053; BAB13053; BAB13053.
DR KEGG; buc:BU348; -.
DR PATRIC; fig|107806.10.peg.360; -.
DR eggNOG; COG0564; Bacteria.
DR eggNOG; COG1188; Bacteria.
DR HOGENOM; CLU_016902_1_1_6; -.
DR OMA; PKTHVYR; -.
DR Proteomes; UP000001806; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..314
FT /note="Ribosomal large subunit pseudouridine synthase C"
FT /id="PRO_0000162664"
FT DOMAIN 20..84
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 143
FT /evidence="ECO:0000250"
SQ SEQUENCE 314 AA; 36614 MW; 4B0FC4D2FD085B08 CRC64;
MTHKILPISI IYINKEMLNQ RIDNFMRSKF KSVPKSMIYR IIRTGKIRIN KKRIKPHYKL
KIGDILKIPP IKILCDIKNT FFPLNHSTNL LNRILYEDSH LLIINKPSGI AVHGGSGLNF
GVIEYFRKLR PLNKFLELVH RIDRDTSGVL ILAKKRTSLV SLHEQLREKK IKKEYIALVH
GLWPDHIKKI SEPLLKIQFK NKQKMVLIDK KGKPSETYFQ IKKKFSSSTL LSIIPKTGRT
HQIRVHALHA GHPIFFDKRY GKNDLDADIK NNHKINRLLL HSSGVHFIHP KNGNKIYIKA
PLDIDFQNYL NTII
