RLUC_BUCAP
ID RLUC_BUCAP Reviewed; 314 AA.
AC Q8K9J8;
DT 15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase C;
DE EC=5.4.99.24;
DE AltName: Full=23S rRNA pseudouridine(955/2504/2580) synthase;
DE AltName: Full=rRNA pseudouridylate synthase C;
DE AltName: Full=rRNA-uridine isomerase C;
GN Name=rluC; OrderedLocusNames=BUsg_336;
OS Buchnera aphidicola subsp. Schizaphis graminum (strain Sg).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Buchnera.
OX NCBI_TaxID=198804;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sg;
RX PubMed=12089438; DOI=10.1126/science.1071278;
RA Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT "50 million years of genomic stasis in endosymbiotic bacteria.";
RL Science 296:2376-2379(2002).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC positions 955, 2504 and 2580 in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(955/2504/2580) in 23S rRNA =
CC pseudouridine(955/2504/2580) in 23S rRNA; Xref=Rhea:RHEA:42528,
CC Rhea:RHEA-COMP:10099, Rhea:RHEA-COMP:10100, ChEBI:CHEBI:65314,
CC ChEBI:CHEBI:65315; EC=5.4.99.24;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
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DR EMBL; AE013218; AAM67890.1; -; Genomic_DNA.
DR RefSeq; WP_011053857.1; NC_004061.1.
DR AlphaFoldDB; Q8K9J8; -.
DR SMR; Q8K9J8; -.
DR STRING; 198804.BUsg_336; -.
DR EnsemblBacteria; AAM67890; AAM67890; BUsg_336.
DR KEGG; bas:BUsg_336; -.
DR eggNOG; COG0564; Bacteria.
DR HOGENOM; CLU_016902_1_1_6; -.
DR OMA; PKTHVYR; -.
DR OrthoDB; 1714699at2; -.
DR Proteomes; UP000000416; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; RNA-binding; rRNA processing.
FT CHAIN 1..314
FT /note="Ribosomal large subunit pseudouridine synthase C"
FT /id="PRO_0000162665"
FT DOMAIN 20..94
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 143
FT /evidence="ECO:0000250"
SQ SEQUENCE 314 AA; 36830 MW; D94B3D06413CEE76 CRC64;
MKYKTTSVSI IYINEDMINQ RIDNFLQKKL KNVPRSMIYR IIRTGKIRIN KKRIKPDYKL
KIGDKLRIPP IKILCEKKSS LLTTEYKKNL LNNILYEDNY LLIINKPSGI AVHGGSGINL
GVIEYFRKIR PLEKFLELVH RIDRDTSGVL MLAKKRRSLL SLHKQIREKK VQKKYIALVH
GLWPLSLRKV SEPLLKTHLK NKQRKILINK EGKPAETYFK IKKQYLFTTL VSITPKTGRT
HQIRVHTLHA GHPILFDKRY GKRDLDCNIK NKTKINRLLL HATSINFIHP ETGKKMHIVA
PLDVYFKNYL NTLI
