RLUC_ECO57
ID RLUC_ECO57 Reviewed; 319 AA.
AC Q8X8J3;
DT 29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase C;
DE EC=5.4.99.24;
DE AltName: Full=23S rRNA pseudouridine(955/2504/2580) synthase;
DE AltName: Full=rRNA pseudouridylate synthase C;
DE AltName: Full=rRNA-uridine isomerase C;
GN Name=rluC; OrderedLocusNames=Z1725, ECs1464;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC positions 955, 2504 and 2580 in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(955/2504/2580) in 23S rRNA =
CC pseudouridine(955/2504/2580) in 23S rRNA; Xref=Rhea:RHEA:42528,
CC Rhea:RHEA-COMP:10099, Rhea:RHEA-COMP:10100, ChEBI:CHEBI:65314,
CC ChEBI:CHEBI:65315; EC=5.4.99.24;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
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DR EMBL; AE005174; AAG55832.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB34887.1; -; Genomic_DNA.
DR PIR; D85671; D85671.
DR PIR; H90811; H90811.
DR RefSeq; NP_309491.1; NC_002695.1.
DR RefSeq; WP_000846342.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; Q8X8J3; -.
DR SMR; Q8X8J3; -.
DR STRING; 155864.EDL933_1663; -.
DR EnsemblBacteria; AAG55832; AAG55832; Z1725.
DR EnsemblBacteria; BAB34887; BAB34887; ECs_1464.
DR GeneID; 913470; -.
DR KEGG; ece:Z1725; -.
DR KEGG; ecs:ECs_1464; -.
DR PATRIC; fig|386585.9.peg.1564; -.
DR eggNOG; COG0564; Bacteria.
DR HOGENOM; CLU_016902_1_1_6; -.
DR OMA; PKTHVYR; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..319
FT /note="Ribosomal large subunit pseudouridine synthase C"
FT /id="PRO_0000162669"
FT DOMAIN 20..83
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 144
FT /evidence="ECO:0000250"
SQ SEQUENCE 319 AA; 36013 MW; 19C8D05E426E4644 CRC64;
MKTETPSVKI VAITADEAGQ RIDNFLRTQL KGVPKSMIYR ILRKGEVRVN KKRIKPEYKL
EAGDEVRIPP VRVAEREEEA VSPHLQKVAA LADVILYEDD HILVLNKPSG TAVHGGSGLS
FGVIEGLRAL RPEARFLELV HRLDRDTSGV LLVAKKRSAL RSLHEQLREK GMQKDYLALV
RGQWQSHVKS VQAPLLKNIL QSGERIVRVS QEGKPSETRF KVEERYAFAT LVRCSPVTGR
THQIRVHTQY AGHPIAFDDR YGDREFDRQL TEAGTGLNRL FLHAAALKFT HPGTGEVMRI
EAPMDDGLKR CLQKLRNAR
