RLUC_PSEAE
ID RLUC_PSEAE Reviewed; 318 AA.
AC Q9HZM9;
DT 29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase C;
DE EC=5.4.99.24;
DE AltName: Full=23S rRNA pseudouridine(955/2504/2580) synthase;
DE AltName: Full=rRNA pseudouridylate synthase C;
DE AltName: Full=rRNA-uridine isomerase C;
GN Name=rluC; OrderedLocusNames=PA2975;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC positions 955, 2504 and 2580 in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(955/2504/2580) in 23S rRNA =
CC pseudouridine(955/2504/2580) in 23S rRNA; Xref=Rhea:RHEA:42528,
CC Rhea:RHEA-COMP:10099, Rhea:RHEA-COMP:10100, ChEBI:CHEBI:65314,
CC ChEBI:CHEBI:65315; EC=5.4.99.24;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
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DR EMBL; AE004091; AAG06363.1; -; Genomic_DNA.
DR PIR; G83273; G83273.
DR RefSeq; NP_251665.1; NC_002516.2.
DR RefSeq; WP_003104143.1; NZ_QZGE01000009.1.
DR AlphaFoldDB; Q9HZM9; -.
DR SMR; Q9HZM9; -.
DR STRING; 287.DR97_4964; -.
DR PaxDb; Q9HZM9; -.
DR PRIDE; Q9HZM9; -.
DR DNASU; 880474; -.
DR EnsemblBacteria; AAG06363; AAG06363; PA2975.
DR GeneID; 880474; -.
DR KEGG; pae:PA2975; -.
DR PATRIC; fig|208964.12.peg.3121; -.
DR PseudoCAP; PA2975; -.
DR HOGENOM; CLU_016902_1_1_6; -.
DR InParanoid; Q9HZM9; -.
DR OMA; PKTHVYR; -.
DR PhylomeDB; Q9HZM9; -.
DR BioCyc; PAER208964:G1FZ6-3027-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..318
FT /note="Ribosomal large subunit pseudouridine synthase C"
FT /id="PRO_0000162673"
FT DOMAIN 23..83
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 146
FT /evidence="ECO:0000250"
SQ SEQUENCE 318 AA; 35641 MW; 354B265A4C5C605E CRC64;
MTTPASPTTG VQLLDVPPEL AGQRIDNFLI ARLKGAPKTL IYRILRKGEV RVNKGRIKPD
YRLQAGDQVR VPPLRLAERD EPAPLAQGLL ERMEAAIVYE DKALIVVNKP AGIAVHGGSG
VSAGVIEVFR QLRPDAKDIE LVHRLDRDTS GLLMIAKKRS MLRHLHEALR GEKIVDKRYH
ALVRGHWPAG KKNVRAPLLK NNLRSGERMV EVNVEGKDAL TEFRVLRRFG DFATLVEARP
ITGRTHQIRV HAKHAGHAIA GDPKYGDEDF SREIRELGGK RLFLHAAQLR VPLPDGQVLE
LEAPVDEMWQ RSLERLND
