ID   RLUC_VIBCH              Reviewed;         315 AA.
AC   Q9KQH0;
DT   20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase C;
DE            EC=5.4.99.24;
DE   AltName: Full=23S rRNA pseudouridine(955/2504/2580) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase C;
DE   AltName: Full=rRNA-uridine isomerase C;
GN   Name=rluC; OrderedLocusNames=VC_2028;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC       positions 955, 2504 and 2580 in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(955/2504/2580) in 23S rRNA =
CC         pseudouridine(955/2504/2580) in 23S rRNA; Xref=Rhea:RHEA:42528,
CC         Rhea:RHEA-COMP:10099, Rhea:RHEA-COMP:10100, ChEBI:CHEBI:65314,
CC         ChEBI:CHEBI:65315; EC=5.4.99.24;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; AE003852; AAF95176.1; -; Genomic_DNA.
DR   PIR; G82126; G82126.
DR   RefSeq; NP_231662.1; NC_002505.1.
DR   RefSeq; WP_001002871.1; NZ_LT906614.1.
DR   AlphaFoldDB; Q9KQH0; -.
DR   SMR; Q9KQH0; -.
DR   STRING; 243277.VC_2028; -.
DR   DNASU; 2613407; -.
DR   EnsemblBacteria; AAF95176; AAF95176; VC_2028.
DR   GeneID; 57740655; -.
DR   KEGG; vch:VC_2028; -.
DR   PATRIC; fig|243277.26.peg.1938; -.
DR   eggNOG; COG0564; Bacteria.
DR   HOGENOM; CLU_016902_1_1_6; -.
DR   OMA; PKTHVYR; -.
DR   BioCyc; VCHO:VC2028-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT   CHAIN           1..315
FT                   /note="Ribosomal large subunit pseudouridine synthase C"
FT                   /id="PRO_0000162679"
FT   DOMAIN          20..94
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        146
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   315 AA;  35996 MW;  171DE0BEC8948687 CRC64;
     MNEIRTQVQF IDIDEDMAGQ RIDNFLRNQL KNIPKSVVYR ILRKGEVRVN KKRVKAEYKL
     EAGDVVRVPP VTVEVKENEP APPSTKLSKV AELEHCIVYE DDHLLILNKP SGTAVHGGSG
     LHFGAIEALR ALRPQARFLE LVHRIDRDTS GILLVAKKRS ALRHLQAQFR EKTVQKYYYA
     LVMGHWDAEC KVVNAPLLKN EVNSIVRVNP NGKPSETRFR ILEKFAEATL VQASPVTGRT
     HQIRVHTQYM GHPIAWDDRY GDRRFDAYTA QFGIERLFLH AANIQFVHPA SEKKLEIHAP
     LEAHLEQALT KMRQA