RLUC_VIBPA
ID RLUC_VIBPA Reviewed; 315 AA.
AC Q87N15;
DT 29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase C;
DE EC=5.4.99.24;
DE AltName: Full=23S rRNA pseudouridine(955/2504/2580) synthase;
DE AltName: Full=rRNA pseudouridylate synthase C;
DE AltName: Full=rRNA-uridine isomerase C;
GN Name=rluC; OrderedLocusNames=VP2061;
OS Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=223926;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIMD 2210633;
RX PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT distinct from that of V. cholerae.";
RL Lancet 361:743-749(2003).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC positions 955, 2504 and 2580 in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(955/2504/2580) in 23S rRNA =
CC pseudouridine(955/2504/2580) in 23S rRNA; Xref=Rhea:RHEA:42528,
CC Rhea:RHEA-COMP:10099, Rhea:RHEA-COMP:10100, ChEBI:CHEBI:65314,
CC ChEBI:CHEBI:65315; EC=5.4.99.24;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
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DR EMBL; BA000031; BAC60324.1; -; Genomic_DNA.
DR RefSeq; NP_798440.1; NC_004603.1.
DR RefSeq; WP_005461586.1; NC_004603.1.
DR AlphaFoldDB; Q87N15; -.
DR SMR; Q87N15; -.
DR STRING; 223926.28807054; -.
DR EnsemblBacteria; BAC60324; BAC60324; BAC60324.
DR GeneID; 1189572; -.
DR KEGG; vpa:VP2061; -.
DR PATRIC; fig|223926.6.peg.1971; -.
DR eggNOG; COG0564; Bacteria.
DR HOGENOM; CLU_016902_1_1_6; -.
DR OMA; PKTHVYR; -.
DR Proteomes; UP000002493; Chromosome 1.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..315
FT /note="Ribosomal large subunit pseudouridine synthase C"
FT /id="PRO_0000162680"
FT DOMAIN 20..93
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 145
FT /evidence="ECO:0000250"
SQ SEQUENCE 315 AA; 36142 MW; 48298760D3A743A8 CRC64;
MSEIRTQVQF VDIDEDMAGQ RIDNFLRNQL KDIPKSMIYR IVRKGEVRVN KKRIKAEYKL
KAGDLVRIPP VTVEKKEEDV APSTKLNKVA ELEHMIIYED DHMLILNKPS GTAVHGGSGL
KFGAIEALRA LRPQARFLEL VHRIDRDTSG ILLVAKKRSA LRHLQAQFRE KTVKKFYFAL
VMGQWKSSCK VVNAPLLKNE VNSIVRVNPN GKPSETRFKI LEKFEQATLI QASPITGRTH
QIRVHTQYTG HPIAWDDRYG DRRFDAYTGQ LGLDRLFLHA ANIKFQHPSN DEWMEINAPM
ESKLEKVLVG LRKAN
