RLUD_BLOFL
ID RLUD_BLOFL Reviewed; 321 AA.
AC P59838;
DT 29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 29-AUG-2003, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase D;
DE EC=5.4.99.23;
DE AltName: Full=23S rRNA pseudouridine(1911/1915/1917) synthase;
DE AltName: Full=rRNA pseudouridylate synthase D;
DE AltName: Full=rRNA-uridine isomerase D;
GN Name=rluD; OrderedLocusNames=Bfl181;
OS Blochmannia floridanus.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; ant endosymbionts; Candidatus Blochmannia.
OX NCBI_TaxID=203907;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12886019; DOI=10.1073/pnas.1533499100;
RA Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F.,
RA Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B.,
RA van Ham R.C.H.J., Gross R., Moya A.;
RT "The genome sequence of Blochmannia floridanus: comparative analysis of
RT reduced genomes.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC positions 1911, 1915 and 1917 in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(1911/1915/1917) in 23S rRNA =
CC pseudouridine(1911/1915/1917) in 23S rRNA; Xref=Rhea:RHEA:42524,
CC Rhea:RHEA-COMP:10097, Rhea:RHEA-COMP:10098, ChEBI:CHEBI:65314,
CC ChEBI:CHEBI:65315; EC=5.4.99.23;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
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DR EMBL; BX248583; CAD83700.1; -; Genomic_DNA.
DR RefSeq; WP_011126467.1; NC_005061.1.
DR AlphaFoldDB; P59838; -.
DR SMR; P59838; -.
DR STRING; 203907.Bfl181; -.
DR EnsemblBacteria; CAD83700; CAD83700; Bfl181.
DR KEGG; bfl:Bfl181; -.
DR eggNOG; COG0564; Bacteria.
DR HOGENOM; CLU_016902_4_4_6; -.
DR OMA; AHPSPGW; -.
DR OrthoDB; 1714699at2; -.
DR Proteomes; UP000002192; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..321
FT /note="Ribosomal large subunit pseudouridine synthase D"
FT /id="PRO_0000162687"
FT DOMAIN 16..93
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 142
FT /evidence="ECO:0000250"
SQ SEQUENCE 321 AA; 37066 MW; 46B38256105D11F6 CRC64;
MVIIKPMIVQ VHQSGYRLDY ILSKLLPQYS RSQIKHWILN KKVVVNNQVS TLPRKKVVYG
ELIEIKYINN NDICKSEDIV PQNIPLNIIY EDNDILVINK ASNMVVHPGI GHYQGTVLNA
LLYRYPSILK ISKQAGIVQR LDKDTTGLMI IAKTVSAYDN LLRSFKLRKV VKEYDAIVYG
KFTSNAGVVN QPMRRHFAKR TFMSVHYTGK SAVTYYSVVE EFKAHSRVRI NLKTGRTHQI
RVHMAYINHP LVGDQKYKGN FSVFDIKKMS DELNNYLLDF NRQALHACTL QLLHPITQVQ
MKWNAPLPQD ILQLIAILKK Y
