RLUD_BUCAP
ID RLUD_BUCAP Reviewed; 315 AA.
AC Q8K9E9;
DT 08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase D;
DE EC=5.4.99.23;
DE AltName: Full=23S rRNA pseudouridine(1911/1915/1917) synthase;
DE AltName: Full=rRNA pseudouridylate synthase D;
DE AltName: Full=rRNA-uridine isomerase D;
GN Name=rluD; OrderedLocusNames=BUsg_388;
OS Buchnera aphidicola subsp. Schizaphis graminum (strain Sg).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Buchnera.
OX NCBI_TaxID=198804;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sg;
RX PubMed=12089438; DOI=10.1126/science.1071278;
RA Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT "50 million years of genomic stasis in endosymbiotic bacteria.";
RL Science 296:2376-2379(2002).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC positions 1911, 1915 and 1917 in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(1911/1915/1917) in 23S rRNA =
CC pseudouridine(1911/1915/1917) in 23S rRNA; Xref=Rhea:RHEA:42524,
CC Rhea:RHEA-COMP:10097, Rhea:RHEA-COMP:10098, ChEBI:CHEBI:65314,
CC ChEBI:CHEBI:65315; EC=5.4.99.23;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
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DR EMBL; AE013218; AAM67940.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8K9E9; -.
DR SMR; Q8K9E9; -.
DR STRING; 198804.BUsg_388; -.
DR EnsemblBacteria; AAM67940; AAM67940; BUsg_388.
DR KEGG; bas:BUsg_388; -.
DR eggNOG; COG0564; Bacteria.
DR HOGENOM; CLU_016902_4_0_6; -.
DR OMA; AHPSPGW; -.
DR Proteomes; UP000000416; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; RNA-binding; rRNA processing.
FT CHAIN 1..315
FT /note="Ribosomal large subunit pseudouridine synthase D"
FT /id="PRO_0000162685"
FT DOMAIN 18..87
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 139
FT /evidence="ECO:0000250"
SQ SEQUENCE 315 AA; 36419 MW; 95027F8F82A0B5A5 CRC64;
MEKKELSACV PFMGFSGKRL DQILSKLFMQ YSRSCLKKWI IMNNVYINGK IENQPDKKIL
GGEKITIYST DEKLPINLPQ NIALNTIYED SNILIINKPP GLVVHPGAGN QDGTILNALL
YRYTNISSIP RCGIIHRLDK DTSGLMVIAK TIFSYNKLVT LLKKRKIIRK YQGIIKGNMI
SGGIVNYPIM RHPFKRICMT TDSLGKKSIT HYKIINRFKF HTHVSFRLET GRTHQIRVHM
LHIGYPLLGD PLYGGIDYGC NYFQENRNIY RKFNLFRQAL HADYIEFIHP FTQKVMSWSA
SLPQDMKDIL LYLKK
