RLUD_BUCBP
ID RLUD_BUCBP Reviewed; 317 AA.
AC Q89AD9;
DT 29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase D;
DE EC=5.4.99.23;
DE AltName: Full=23S rRNA pseudouridine(1911/1915/1917) synthase;
DE AltName: Full=rRNA pseudouridylate synthase D;
DE AltName: Full=rRNA-uridine isomerase D;
GN Name=rluD; OrderedLocusNames=bbp_363;
OS Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Buchnera.
OX NCBI_TaxID=224915;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bp;
RX PubMed=12522265; DOI=10.1073/pnas.0235981100;
RA van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F.,
RA Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J.,
RA Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.;
RT "Reductive genome evolution in Buchnera aphidicola.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC positions 1911, 1915 and 1917 in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(1911/1915/1917) in 23S rRNA =
CC pseudouridine(1911/1915/1917) in 23S rRNA; Xref=Rhea:RHEA:42524,
CC Rhea:RHEA-COMP:10097, Rhea:RHEA-COMP:10098, ChEBI:CHEBI:65314,
CC ChEBI:CHEBI:65315; EC=5.4.99.23;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE016826; AAO27082.1; -; Genomic_DNA.
DR RefSeq; WP_011091483.1; NC_004545.1.
DR AlphaFoldDB; Q89AD9; -.
DR SMR; Q89AD9; -.
DR STRING; 224915.bbp_363; -.
DR EnsemblBacteria; AAO27082; AAO27082; bbp_363.
DR GeneID; 56470902; -.
DR KEGG; bab:bbp_363; -.
DR eggNOG; COG0564; Bacteria.
DR HOGENOM; CLU_016902_4_0_6; -.
DR OMA; AHPSPGW; -.
DR OrthoDB; 1714699at2; -.
DR Proteomes; UP000000601; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..317
FT /note="Ribosomal large subunit pseudouridine synthase D"
FT /id="PRO_0000162686"
FT DOMAIN 19..83
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 140
FT /evidence="ECO:0000250"
SQ SEQUENCE 317 AA; 36552 MW; 1000829E90EFC600 CRC64;
MQNKLKLIAI VPKISLLKNR LDQVLASLFK KHSRTILKRH ILSKDVCVNG RILDQPDAKV
LYKDIISINL KFKAKNDYQA ENIFLNVIYE DDDILVINKQ DNFVVHPGAG NTSGTLLNAL
LHRDSTFFNI PRAGIVHRLD KDTTGLMVIA KNNLSYMALI DQIKCKKVIR EYQAIVYGKV
ISGGTIVKSI IRNPIKRTTM IVNKCGKRAI THYRILKRFT HHTHLKIILE TGRTHQIRTH
MLYINFPLVG DKTYGNKFKF YKNISLTLLE QVKKFPRQAL HASRLCLYHP VTKHLMEWNS
TLPRDMLNLI ALLNKNI
