ID   RLUD_HAEDU              Reviewed;         325 AA.
AC   Q9L7A7;
DT   29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   29-AUG-2003, sequence version 2.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase D;
DE            EC=5.4.99.23;
DE   AltName: Full=23S rRNA pseudouridine(1911/1915/1917) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase D;
DE   AltName: Full=rRNA-uridine isomerase D;
GN   Name=rluD; OrderedLocusNames=HD_0469;
OS   Haemophilus ducreyi (strain 35000HP / ATCC 700724).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=233412;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=35000HP / ATCC 700724;
RA   Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L.,
RA   Nguyen D., Wang J., Forst C., Hood L.;
RT   "The complete genome sequence of Haemophilus ducreyi.";
RL   Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-250.
RC   STRAIN=35000HP / ATCC 700724;
RX   PubMed=10735874; DOI=10.1128/jb.182.8.2292-2298.2000;
RA   Sun S., Schilling B., Tarantino L., Tullius M.V., Gibson B.W.,
RA   Munson R.S. Jr.;
RT   "Cloning and characterization of the lipooligosaccharide
RT   galactosyltransferase II gene of Haemophilus ducreyi.";
RL   J. Bacteriol. 182:2292-2298(2000).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC       positions 1911, 1915 and 1917 in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(1911/1915/1917) in 23S rRNA =
CC         pseudouridine(1911/1915/1917) in 23S rRNA; Xref=Rhea:RHEA:42524,
CC         Rhea:RHEA-COMP:10097, Rhea:RHEA-COMP:10098, ChEBI:CHEBI:65314,
CC         ChEBI:CHEBI:65315; EC=5.4.99.23;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; AE017143; AAP95428.1; -; Genomic_DNA.
DR   EMBL; AF224466; AAF32394.1; -; Genomic_DNA.
DR   RefSeq; WP_010944481.1; NC_002940.2.
DR   AlphaFoldDB; Q9L7A7; -.
DR   SMR; Q9L7A7; -.
DR   STRING; 233412.HD_0469; -.
DR   EnsemblBacteria; AAP95428; AAP95428; HD_0469.
DR   KEGG; hdu:HD_0469; -.
DR   eggNOG; COG0564; Bacteria.
DR   HOGENOM; CLU_016902_4_0_6; -.
DR   OMA; AHPSPGW; -.
DR   Proteomes; UP000001022; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT   CHAIN           1..325
FT                   /note="Ribosomal large subunit pseudouridine synthase D"
FT                   /id="PRO_0000162691"
FT   DOMAIN          18..78
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        139
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   325 AA;  36991 MW;  A93C172718672DE7 CRC64;
     MTQQMTLTAE ISADLLGYRL DQALAQLFPD YSRSRIKVWI ENDLVKVNGI IVNRARGKVF
     GGEQIEIQAE IEEEIRFEPQ NIPLNIVHED DDIIIINKPK NLVVHPGAGN PDGTVLNALL
     YYYPPIAEVP RAGIIHRLDK DTTGLMVVAK NIPAQTHLVT ALQKRRITRE YEAIASGIMT
     QGGKVDEPMA RHPTKRTAMA VHPMGKPAIT HYRIMERFRN YTRLRLRLET GRTHQIRVHM
     AHIAHPLLGD QLYGGRPRPP KGSSEAFLTV LRAFQRQALH ATMLRLEHPI NGELIECHAP
     LPADFVELIE ALKTDYQRYK EDLYY