ID   RLUD_HAEIN              Reviewed;         324 AA.
AC   P44445;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase D;
DE            EC=5.4.99.23;
DE   AltName: Full=23S rRNA pseudouridine(1911/1915/1917) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase D;
DE   AltName: Full=rRNA-uridine isomerase D;
GN   Name=rluD; Synonyms=sfhB; OrderedLocusNames=HI_0176;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC       positions 1911, 1915 and 1917 in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(1911/1915/1917) in 23S rRNA =
CC         pseudouridine(1911/1915/1917) in 23S rRNA; Xref=Rhea:RHEA:42524,
CC         Rhea:RHEA-COMP:10097, Rhea:RHEA-COMP:10098, ChEBI:CHEBI:65314,
CC         ChEBI:CHEBI:65315; EC=5.4.99.23;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; L42023; AAC21845.1; -; Genomic_DNA.
DR   PIR; F64144; F64144.
DR   RefSeq; NP_438344.1; NC_000907.1.
DR   RefSeq; WP_005694111.1; NC_000907.1.
DR   AlphaFoldDB; P44445; -.
DR   SMR; P44445; -.
DR   STRING; 71421.HI_0176; -.
DR   EnsemblBacteria; AAC21845; AAC21845; HI_0176.
DR   KEGG; hin:HI_0176; -.
DR   PATRIC; fig|71421.8.peg.180; -.
DR   eggNOG; COG0564; Bacteria.
DR   HOGENOM; CLU_016902_4_0_6; -.
DR   OMA; AHPSPGW; -.
DR   PhylomeDB; P44445; -.
DR   BioCyc; HINF71421:G1GJ1-186-MON; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT   CHAIN           1..324
FT                   /note="Ribosomal large subunit pseudouridine synthase D"
FT                   /id="PRO_0000162692"
FT   DOMAIN          17..90
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        138
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   324 AA;  37110 MW;  35F452DF65FB9145 CRC64;
     MPQITLSAEV QPEQMGQRLD QTLAELFPEY SRSRLKTWIE ADLVKLNDRI TNIPREKVLG
     GERIEIIVEV EDETRFEAEN IPLNIVYEDD DIIVINKPKD LVVHPGAGNP NGTVLNALLY
     HYPPIVEVPR AGIVHRLDKD TTGLMVVAKT IPAQTKLVRD LQKRKITREY EAVASGIMTK
     GGTVDQPMAR HATKRTLMAV HPMGKPAVTH YRIMENYRNY TRLRLRLETG RTHQIRVHMA
     HIAHPLLGDQ TYGGRPRPPK NASEDFMEVL RNFKRQALHA VMLRLAHPIT GEMMEWYAPL
     PDDFVELLNA LKADYLEHQD ELDY