RLUD_NEIMB
ID RLUD_NEIMB Reviewed; 374 AA.
AC Q9K0B0;
DT 29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase D;
DE EC=5.4.99.23;
DE AltName: Full=23S rRNA pseudouridine(1911/1915/1917) synthase;
DE AltName: Full=rRNA pseudouridylate synthase D;
DE AltName: Full=rRNA-uridine isomerase D;
GN Name=rluD; OrderedLocusNames=NMB0704;
OS Neisseria meningitidis serogroup B (strain MC58).
OC Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC Neisseria.
OX NCBI_TaxID=122586;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MC58;
RX PubMed=10710307; DOI=10.1126/science.287.5459.1809;
RA Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E.,
RA Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C.,
RA Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H.,
RA Salzberg S.L., White O., Fleischmann R.D., Dougherty B.A., Mason T.M.,
RA Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D.,
RA Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V.,
RA Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M.,
RA Moxon E.R., Rappuoli R., Venter J.C.;
RT "Complete genome sequence of Neisseria meningitidis serogroup B strain
RT MC58.";
RL Science 287:1809-1815(2000).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC positions 1911, 1915 and 1917 in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(1911/1915/1917) in 23S rRNA =
CC pseudouridine(1911/1915/1917) in 23S rRNA; Xref=Rhea:RHEA:42524,
CC Rhea:RHEA-COMP:10097, Rhea:RHEA-COMP:10098, ChEBI:CHEBI:65314,
CC ChEBI:CHEBI:65315; EC=5.4.99.23;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
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DR EMBL; AE002098; AAF41121.1; -; Genomic_DNA.
DR PIR; A81168; A81168.
DR RefSeq; NP_273746.1; NC_003112.2.
DR RefSeq; WP_002247551.1; NC_003112.2.
DR AlphaFoldDB; Q9K0B0; -.
DR SMR; Q9K0B0; -.
DR STRING; 122586.NMB0704; -.
DR PaxDb; Q9K0B0; -.
DR EnsemblBacteria; AAF41121; AAF41121; NMB0704.
DR KEGG; nme:NMB0704; -.
DR PATRIC; fig|122586.8.peg.896; -.
DR HOGENOM; CLU_016902_4_0_4; -.
DR OMA; AHPSPGW; -.
DR Proteomes; UP000000425; Chromosome.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..374
FT /note="Ribosomal large subunit pseudouridine synthase D"
FT /id="PRO_0000162694"
FT DOMAIN 39..112
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 160
FT /evidence="ECO:0000250"
SQ SEQUENCE 374 AA; 41453 MW; E5648CACB9A1A9DF CRC64;
MQNTSFDNES DYSDDSDFAS ASETENRIGL TVPLELAGGR LDAVLAKLLP DYSRSRLTSW
IKEGAVIVND KPSQPKDKMI GGEQICVTVR PSEENLAFVP EPMALDIVYE DDTVIVVNKP
AGLVVHPAAG NWTGTLLNGL LAHCPELSQV PRAGIVHRLD KETSGLMVVA KTLPAQNSLV
RQLQERTVKR IYRAVANGIV PFDGKIETQI GRDPHNRLKM AVVKFGGKPA VTHVKVLERY
LTHSYIECSL ETGRTHQIRV HMREANHPLA ADPVYGNPRH PCGDTVKEAV KSLGARQALH
AYRLSFTHPE SGETVSFEAP IPNDIYHLLS VLRLEAGLDS SLSNEEEWQD KFGADDDDDW
NEDDYDVEVV YVRE