RLUD_SALTY
ID RLUD_SALTY Reviewed; 326 AA.
AC P65836; Q8XGG2;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase D;
DE EC=5.4.99.23;
DE AltName: Full=23S rRNA pseudouridine(1911/1915/1917) synthase;
DE AltName: Full=rRNA pseudouridylate synthase D;
DE AltName: Full=rRNA-uridine isomerase D;
GN Name=rluD; Synonyms=sfhB; OrderedLocusNames=STM2662;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND INTERACTION WITH OBG/YHBZ.
RC STRAIN=FIRN / SL3261;
RX PubMed=17905831; DOI=10.1110/ps.072900907;
RA Lamb H.K., Thompson P., Elliott C., Charles I.G., Richards J., Lockyer M.,
RA Watkins N., Nichols C., Stammers D.K., Bagshaw C.R., Cooper A.,
RA Hawkins A.R.;
RT "Functional analysis of the GTPases EngA and YhbZ encoded by Salmonella
RT typhimurium.";
RL Protein Sci. 16:2391-2402(2007).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC positions 1911, 1915 and 1917 in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(1911/1915/1917) in 23S rRNA =
CC pseudouridine(1911/1915/1917) in 23S rRNA; Xref=Rhea:RHEA:42524,
CC Rhea:RHEA-COMP:10097, Rhea:RHEA-COMP:10098, ChEBI:CHEBI:65314,
CC ChEBI:CHEBI:65315; EC=5.4.99.23;
CC -!- SUBUNIT: Binds Obg/YhbZ (AC Q8ZLS5).
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
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DR EMBL; AE006468; AAL21552.1; -; Genomic_DNA.
DR RefSeq; NP_461593.1; NC_003197.2.
DR RefSeq; WP_000079130.1; NC_003197.2.
DR AlphaFoldDB; P65836; -.
DR SMR; P65836; -.
DR STRING; 99287.STM2662; -.
DR PaxDb; P65836; -.
DR EnsemblBacteria; AAL21552; AAL21552; STM2662.
DR GeneID; 1254185; -.
DR KEGG; stm:STM2662; -.
DR PATRIC; fig|99287.12.peg.2808; -.
DR HOGENOM; CLU_016902_4_0_6; -.
DR OMA; AHPSPGW; -.
DR PhylomeDB; P65836; -.
DR BioCyc; SENT99287:STM2662-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 1: Evidence at protein level;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..326
FT /note="Ribosomal large subunit pseudouridine synthase D"
FT /id="PRO_0000162699"
FT DOMAIN 18..91
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT REGION 183..203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 139
FT /evidence="ECO:0000250"
SQ SEQUENCE 326 AA; 37100 MW; 412704BF0F6E7098 CRC64;
MAQRVQLTAT VSENQLGQRL DQALAEMFPD YSRSRIKEWI LNQRVLVNGQ LCDKPKEKVL
GGERVAIDAE IDEEIRFEAQ DIPLDIVYED DDILVINKPR DLVVHPGAGN PDGTVLNALL
HYYPPIADVP RAGIVHRLDK DTTGLMVVAK TVPAQTRLVE SLQLREITRE YEAVAIGHMT
AGGTVNEPIS RHPTKRTHMS VHPMGKPAVT HYRIMEHFRV HTRLRLRLET GRTHQIRVHM
AHITHPLVGD QVYGGRPRPP KGASEEFIST LRKFDRQALH ATMLRLYHPV SGIEMEWHAP
IPQDMVDLID AMRADFEDHK DDVDWL
