ID   RLUD_VIBCH              Reviewed;         324 AA.
AC   Q9KU20;
DT   29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase D;
DE            EC=5.4.99.23;
DE   AltName: Full=23S rRNA pseudouridine(1911/1915/1917) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase D;
DE   AltName: Full=rRNA-uridine isomerase D;
GN   Name=rluD; OrderedLocusNames=VC_0709;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC       positions 1911, 1915 and 1917 in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(1911/1915/1917) in 23S rRNA =
CC         pseudouridine(1911/1915/1917) in 23S rRNA; Xref=Rhea:RHEA:42524,
CC         Rhea:RHEA-COMP:10097, Rhea:RHEA-COMP:10098, ChEBI:CHEBI:65314,
CC         ChEBI:CHEBI:65315; EC=5.4.99.23;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; AE003852; AAF93874.1; -; Genomic_DNA.
DR   PIR; G82289; G82289.
DR   RefSeq; NP_230358.1; NC_002505.1.
DR   RefSeq; WP_000941107.1; NZ_LT906614.1.
DR   AlphaFoldDB; Q9KU20; -.
DR   SMR; Q9KU20; -.
DR   STRING; 243277.VC_0709; -.
DR   PRIDE; Q9KU20; -.
DR   DNASU; 2615713; -.
DR   EnsemblBacteria; AAF93874; AAF93874; VC_0709.
DR   KEGG; vch:VC_0709; -.
DR   PATRIC; fig|243277.26.peg.679; -.
DR   eggNOG; COG0564; Bacteria.
DR   HOGENOM; CLU_016902_4_0_6; -.
DR   OMA; AHPSPGW; -.
DR   BioCyc; VCHO:VC0709-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT   CHAIN           1..324
FT                   /note="Ribosomal large subunit pseudouridine synthase D"
FT                   /id="PRO_0000162702"
FT   DOMAIN          18..91
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        139
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   324 AA;  36742 MW;  D702C1EF1258E603 CRC64;
     MAHQIELTQT VKDSQLGQRL DQAVAELFTD FSRSRLKEWL LEGKIAVNGD VITKPRTKVM
     GGEVITVQAE LEDEQRWEAQ DLPLNIVYED DDIIVINKPR DFVVHPGAGQ ADKTVLNALL
     FHYSPIAEVP RAGIVHRLDK DTTGLMVVAK TVPAQTRLVR ALQKRDVTRE YEAIVIGTMT
     AGGMIDKPIG RHSTKRTLMS VSPMGKHAVT HYRVAEHFRE HTRLRLRLET GRTHQIRVHM
     AYLQHPLLGD TAYGGRARIP KGATEELTEM IRDFDRQALH AVMLKFEHPV TGEELEFHAP
     VPDDMVEMTL ALREDAKLNR TEEY