ID   RLUD_ZYMMO              Reviewed;         317 AA.
AC   P50513; Q5NPI6;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 2.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase D;
DE            EC=5.4.99.23;
DE   AltName: Full=23S rRNA pseudouridine(1911/1915/1917) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase D;
DE   AltName: Full=rRNA-uridine isomerase D;
GN   Name=rluD; OrderedLocusNames=ZMO0750;
OS   Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Zymomonadaceae; Zymomonas.
OX   NCBI_TaxID=264203;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 31821 / ZM4 / CP4;
RX   PubMed=7501460;
RA   Nakahigashi K., Yanagi H., Yura T.;
RT   "Isolation and sequence analysis of rpoH genes encoding sigma 32 homologs
RT   from Gram-negative bacteria: conserved mRNA and protein segments for heat
RT   shock regulation.";
RL   Nucleic Acids Res. 23:4383-4390(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31821 / ZM4 / CP4;
RX   PubMed=15592456; DOI=10.1038/nbt1045;
RA   Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H.,
RA   Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J.,
RA   Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y., Kang H.L., Lee S.Y., Lee K.J.,
RA   Kang H.S.;
RT   "The genome sequence of the ethanologenic bacterium Zymomonas mobilis
RT   ZM4.";
RL   Nat. Biotechnol. 23:63-68(2005).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC       positions 1911, 1915 and 1917 in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(1911/1915/1917) in 23S rRNA =
CC         pseudouridine(1911/1915/1917) in 23S rRNA; Xref=Rhea:RHEA:42524,
CC         Rhea:RHEA-COMP:10097, Rhea:RHEA-COMP:10098, ChEBI:CHEBI:65314,
CC         ChEBI:CHEBI:65315; EC=5.4.99.23;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; D50832; BAA09443.1; -; Genomic_DNA.
DR   EMBL; AE008692; AAV89374.1; -; Genomic_DNA.
DR   PIR; S60168; S60168.
DR   RefSeq; WP_011240631.1; NZ_CP035711.1.
DR   AlphaFoldDB; P50513; -.
DR   SMR; P50513; -.
DR   STRING; 264203.ZMO0750; -.
DR   EnsemblBacteria; AAV89374; AAV89374; ZMO0750.
DR   GeneID; 58026569; -.
DR   KEGG; zmo:ZMO0750; -.
DR   eggNOG; COG0564; Bacteria.
DR   HOGENOM; CLU_016902_4_1_5; -.
DR   OMA; AHPSPGW; -.
DR   OrthoDB; 1714699at2; -.
DR   Proteomes; UP000001173; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT   CHAIN           1..317
FT                   /note="Ribosomal large subunit pseudouridine synthase D"
FT                   /id="PRO_0000162710"
FT   DOMAIN          15..89
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        141
FT                   /evidence="ECO:0000250"
FT   CONFLICT        180
FT                   /note="H -> N (in Ref. 1; BAA09443)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        217
FT                   /note="R -> C (in Ref. 1; BAA09443)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   317 AA;  34862 MW;  34960C6B7C28E29F CRC64;
     MPTEQVTLTE EMIGWRLDRA LASLITRLSR ERLKNLISSG CVSNSQGALV RDPAFKIKSL
     DCFTVDIPLP RPAHNEPQDI PLEIVFEDEH LLVVNKPAGM VVHPAAGNYD NTLVNALLYH
     CAGKLSGIGG VARPGIVHRI DKDTSGLLVV AKTDPAHAGL AAQFADHSIN RRYRAIVDGH
     PSLQGHVDAP LARSSVNRQK MAIVSDGRGK RAVTHYRMIT PLKNASLIEC RLETGRTHQV
     RVHMSSIGHS LLGDPVYGRS KKAHHALLQS LAFQRQALHA AHLGFIHPIS GKQVDFDAEM
     PQDMQLLFKM LMISNRN