RLUE_ECO57
ID RLUE_ECO57 Reviewed; 217 AA.
AC Q8X724;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase E;
DE EC=5.4.99.20;
DE AltName: Full=rRNA pseudouridylate synthase E;
DE AltName: Full=rRNA-uridine isomerase E;
GN Name=rluE; OrderedLocusNames=Z1864, ECs1607;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2457
CC in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(2457) in 23S rRNA = pseudouridine(2457) in 23S rRNA;
CC Xref=Rhea:RHEA:38871, Rhea:RHEA-COMP:10091, Rhea:RHEA-COMP:10092,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.20;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG55961.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE005174; AAG55961.1; ALT_INIT; Genomic_DNA.
DR EMBL; BA000007; BAB35030.1; -; Genomic_DNA.
DR PIR; E85687; E85687.
DR PIR; G90829; G90829.
DR RefSeq; NP_309634.3; NC_002695.1.
DR RefSeq; WP_001248690.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; Q8X724; -.
DR SMR; Q8X724; -.
DR STRING; 155864.EDL933_1804; -.
DR EnsemblBacteria; AAG55961; AAG55961; Z1864.
DR EnsemblBacteria; BAB35030; BAB35030; ECs_1607.
DR GeneID; 913282; -.
DR KEGG; ece:Z1864; -.
DR KEGG; ecs:ECs_1607; -.
DR PATRIC; fig|386585.9.peg.1707; -.
DR eggNOG; COG1187; Bacteria.
DR HOGENOM; CLU_024979_8_0_6; -.
DR OMA; HPRTYWV; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.1560; -; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; rRNA processing.
FT CHAIN 1..217
FT /note="Ribosomal large subunit pseudouridine synthase E"
FT /id="PRO_0000100003"
FT REGION 19..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 79
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 217 AA; 24866 MW; F677B8DEDC5FD3F6 CRC64;
MRQFIISENT MQKTSFRNHQ VKRFSSQRST RRKPENQPTR VILFNKPYDV LPQFTDEAGR
KTLKEFIPVQ GVYAAGRLDR DSEGLLVLTN NGALQARLTQ PGKRTGKIYY VQVEGIPTQD
ALEALRNGVT LNDGPTLPAG AELVDEPAWL WPRNPPIRER KSIPTSWLKI TLYEGRNRQV
RRMTAHVGFP TLRLIRYAMG DYSLDNLANG EWREATE
