ID   RLUE_ECOL6              Reviewed;         217 AA.
AC   Q8FIB6;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   15-AUG-2003, sequence version 2.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase E;
DE            EC=5.4.99.20;
DE   AltName: Full=rRNA pseudouridylate synthase E;
DE   AltName: Full=rRNA-uridine isomerase E;
GN   Name=rluE; OrderedLocusNames=c1514;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2457
CC       in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2457) in 23S rRNA = pseudouridine(2457) in 23S rRNA;
CC         Xref=Rhea:RHEA:38871, Rhea:RHEA-COMP:10091, Rhea:RHEA-COMP:10092,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.20;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN79983.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AE014075; AAN79983.1; ALT_FRAME; Genomic_DNA.
DR   RefSeq; WP_001248695.1; NZ_CP051263.1.
DR   AlphaFoldDB; Q8FIB6; -.
DR   SMR; Q8FIB6; -.
DR   STRING; 199310.c1514; -.
DR   EnsemblBacteria; AAN79983; AAN79983; c1514.
DR   KEGG; ecc:c1514; -.
DR   eggNOG; COG1187; Bacteria.
DR   HOGENOM; CLU_024979_8_2_6; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
PE   3: Inferred from homology;
KW   Isomerase; rRNA processing.
FT   CHAIN           1..217
FT                   /note="Ribosomal large subunit pseudouridine synthase E"
FT                   /id="PRO_0000100002"
FT   ACT_SITE        79
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   217 AA;  24852 MW;  F683FDDE281AD3F6 CRC64;
     MRQFIISENT MQKTSFRNHQ VKRFSSQRST RRKPENQPTR VILFNKPYDV LPQFTDEAGR
     KTLKEFIPVQ GVYAAGRLDR DSEGLLVLTN NGALQARLTQ PGKRTGKIYY VQVEGIPTQD
     ALEALRNGVT LNDGPTLPAG AELVEEPAWL WPRNPPIRER KSIPTSWLKI TLYEGRNRQV
     RRMTAHVGFP TLRLIRYAMG DYSLDNLANG EWRDATD