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RLUE_ECOLI
ID   RLUE_ECOLI              Reviewed;         217 AA.
AC   P75966;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 2.
DT   03-AUG-2022, entry version 154.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase E;
DE            EC=5.4.99.20;
DE   AltName: Full=rRNA pseudouridylate synthase E;
DE   AltName: Full=rRNA-uridine isomerase E;
GN   Name=rluE; Synonyms=ymfC; OrderedLocusNames=b1135, JW1121;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   FUNCTION, CATALYTIC ACTIVITY, AND MUTAGENESIS OF ASP-79.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=11720289;
RA   Del Campo M., Kaya Y., Ofengand J.;
RT   "Identification and site of action of the remaining four putative
RT   pseudouridine synthases in Escherichia coli.";
RL   RNA 7:1603-1615(2001).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS) OF 29-217, AND ACTIVE SITE.
RX   PubMed=17320904; DOI=10.1016/j.jmb.2007.01.084;
RA   Pan H., Ho J.D., Stroud R.M., Finer-Moore J.;
RT   "The crystal structure of E. coli rRNA pseudouridine synthase RluE.";
RL   J. Mol. Biol. 367:1459-1470(2007).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2457
CC       in 23S ribosomal RNA. {ECO:0000269|PubMed:11720289}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2457) in 23S rRNA = pseudouridine(2457) in 23S rRNA;
CC         Xref=Rhea:RHEA:38871, Rhea:RHEA-COMP:10091, Rhea:RHEA-COMP:10092,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.20;
CC         Evidence={ECO:0000269|PubMed:11720289};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
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DR   EMBL; U00096; AAC74219.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35957.1; -; Genomic_DNA.
DR   PIR; D64858; D64858.
DR   RefSeq; NP_415653.4; NC_000913.3.
DR   RefSeq; WP_001248691.1; NZ_STEB01000016.1.
DR   PDB; 2OLW; X-ray; 1.60 A; A/B=1-217.
DR   PDB; 2OML; X-ray; 1.20 A; A=29-217.
DR   PDBsum; 2OLW; -.
DR   PDBsum; 2OML; -.
DR   AlphaFoldDB; P75966; -.
DR   SMR; P75966; -.
DR   BioGRID; 4263127; 32.
DR   BioGRID; 850073; 1.
DR   DIP; DIP-12716N; -.
DR   IntAct; P75966; 8.
DR   STRING; 511145.b1135; -.
DR   jPOST; P75966; -.
DR   PaxDb; P75966; -.
DR   PRIDE; P75966; -.
DR   EnsemblBacteria; AAC74219; AAC74219; b1135.
DR   EnsemblBacteria; BAA35957; BAA35957; BAA35957.
DR   GeneID; 66670598; -.
DR   GeneID; 945701; -.
DR   KEGG; ecj:JW1121; -.
DR   KEGG; eco:b1135; -.
DR   PATRIC; fig|1411691.4.peg.1131; -.
DR   EchoBASE; EB3221; -.
DR   eggNOG; COG1187; Bacteria.
DR   HOGENOM; CLU_024979_8_0_6; -.
DR   InParanoid; P75966; -.
DR   OMA; HPRTYWV; -.
DR   PhylomeDB; P75966; -.
DR   BioCyc; EcoCyc:G6581-MON; -.
DR   BioCyc; MetaCyc:G6581-MON; -.
DR   BRENDA; 5.4.99.20; 2026.
DR   BRENDA; 5.4.99.B22; 2026.
DR   EvolutionaryTrace; P75966; -.
DR   PRO; PR:P75966; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IMP:EcoCyc.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IDA:EcoCyc.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IMP:EcoCyc.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Isomerase; Reference proteome; rRNA processing.
FT   CHAIN           1..217
FT                   /note="Ribosomal large subunit pseudouridine synthase E"
FT                   /id="PRO_0000100001"
FT   REGION          19..38
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        79
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000305|PubMed:17320904"
FT   MUTAGEN         79
FT                   /note="D->N,T: Loss of activity."
FT                   /evidence="ECO:0000269|PubMed:11720289"
FT   STRAND          41..46
FT                   /evidence="ECO:0007829|PDB:2OML"
FT   HELIX           63..65
FT                   /evidence="ECO:0007829|PDB:2OML"
FT   STRAND          73..76
FT                   /evidence="ECO:0007829|PDB:2OML"
FT   STRAND          83..90
FT                   /evidence="ECO:0007829|PDB:2OML"
FT   HELIX           92..99
FT                   /evidence="ECO:0007829|PDB:2OML"
FT   STRAND          107..115
FT                   /evidence="ECO:0007829|PDB:2OML"
FT   HELIX           119..127
FT                   /evidence="ECO:0007829|PDB:2OML"
FT   STRAND          140..144
FT                   /evidence="ECO:0007829|PDB:2OML"
FT   STRAND          165..173
FT                   /evidence="ECO:0007829|PDB:2OML"
FT   HELIX           179..186
FT                   /evidence="ECO:0007829|PDB:2OML"
FT   STRAND          191..199
FT                   /evidence="ECO:0007829|PDB:2OML"
FT   STRAND          212..214
FT                   /evidence="ECO:0007829|PDB:2OML"
SQ   SEQUENCE   217 AA;  24880 MW;  F7C7A7CEDC5FD3F6 CRC64;
     MRQFIISENT MQKTSFRNHQ VKRFSSQRST RRKPENQPTR VILFNKPYDV LPQFTDEAGR
     KTLKEFIPVQ GVYAAGRLDR DSEGLLVLTN NGALQARLTQ PGKRTGKIYY VQVEGIPTQD
     ALEALRNGVT LNDGPTLPAG AELVDEPAWL WPRNPPIRER KSIPTSWLKI TLYEGRNRQV
     RRMTAHVGFP TLRLIRYAMG DYSLDNLANG EWREVTD
 
 
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