RLUE_ECOLI
ID RLUE_ECOLI Reviewed; 217 AA.
AC P75966;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase E;
DE EC=5.4.99.20;
DE AltName: Full=rRNA pseudouridylate synthase E;
DE AltName: Full=rRNA-uridine isomerase E;
GN Name=rluE; Synonyms=ymfC; OrderedLocusNames=b1135, JW1121;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, CATALYTIC ACTIVITY, AND MUTAGENESIS OF ASP-79.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=11720289;
RA Del Campo M., Kaya Y., Ofengand J.;
RT "Identification and site of action of the remaining four putative
RT pseudouridine synthases in Escherichia coli.";
RL RNA 7:1603-1615(2001).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS) OF 29-217, AND ACTIVE SITE.
RX PubMed=17320904; DOI=10.1016/j.jmb.2007.01.084;
RA Pan H., Ho J.D., Stroud R.M., Finer-Moore J.;
RT "The crystal structure of E. coli rRNA pseudouridine synthase RluE.";
RL J. Mol. Biol. 367:1459-1470(2007).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2457
CC in 23S ribosomal RNA. {ECO:0000269|PubMed:11720289}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(2457) in 23S rRNA = pseudouridine(2457) in 23S rRNA;
CC Xref=Rhea:RHEA:38871, Rhea:RHEA-COMP:10091, Rhea:RHEA-COMP:10092,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.20;
CC Evidence={ECO:0000269|PubMed:11720289};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
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DR EMBL; U00096; AAC74219.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA35957.1; -; Genomic_DNA.
DR PIR; D64858; D64858.
DR RefSeq; NP_415653.4; NC_000913.3.
DR RefSeq; WP_001248691.1; NZ_STEB01000016.1.
DR PDB; 2OLW; X-ray; 1.60 A; A/B=1-217.
DR PDB; 2OML; X-ray; 1.20 A; A=29-217.
DR PDBsum; 2OLW; -.
DR PDBsum; 2OML; -.
DR AlphaFoldDB; P75966; -.
DR SMR; P75966; -.
DR BioGRID; 4263127; 32.
DR BioGRID; 850073; 1.
DR DIP; DIP-12716N; -.
DR IntAct; P75966; 8.
DR STRING; 511145.b1135; -.
DR jPOST; P75966; -.
DR PaxDb; P75966; -.
DR PRIDE; P75966; -.
DR EnsemblBacteria; AAC74219; AAC74219; b1135.
DR EnsemblBacteria; BAA35957; BAA35957; BAA35957.
DR GeneID; 66670598; -.
DR GeneID; 945701; -.
DR KEGG; ecj:JW1121; -.
DR KEGG; eco:b1135; -.
DR PATRIC; fig|1411691.4.peg.1131; -.
DR EchoBASE; EB3221; -.
DR eggNOG; COG1187; Bacteria.
DR HOGENOM; CLU_024979_8_0_6; -.
DR InParanoid; P75966; -.
DR OMA; HPRTYWV; -.
DR PhylomeDB; P75966; -.
DR BioCyc; EcoCyc:G6581-MON; -.
DR BioCyc; MetaCyc:G6581-MON; -.
DR BRENDA; 5.4.99.20; 2026.
DR BRENDA; 5.4.99.B22; 2026.
DR EvolutionaryTrace; P75966; -.
DR PRO; PR:P75966; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009982; F:pseudouridine synthase activity; IMP:EcoCyc.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IDA:EcoCyc.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IMP:EcoCyc.
DR Gene3D; 3.30.70.1560; -; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Isomerase; Reference proteome; rRNA processing.
FT CHAIN 1..217
FT /note="Ribosomal large subunit pseudouridine synthase E"
FT /id="PRO_0000100001"
FT REGION 19..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 79
FT /note="Nucleophile"
FT /evidence="ECO:0000305|PubMed:17320904"
FT MUTAGEN 79
FT /note="D->N,T: Loss of activity."
FT /evidence="ECO:0000269|PubMed:11720289"
FT STRAND 41..46
FT /evidence="ECO:0007829|PDB:2OML"
FT HELIX 63..65
FT /evidence="ECO:0007829|PDB:2OML"
FT STRAND 73..76
FT /evidence="ECO:0007829|PDB:2OML"
FT STRAND 83..90
FT /evidence="ECO:0007829|PDB:2OML"
FT HELIX 92..99
FT /evidence="ECO:0007829|PDB:2OML"
FT STRAND 107..115
FT /evidence="ECO:0007829|PDB:2OML"
FT HELIX 119..127
FT /evidence="ECO:0007829|PDB:2OML"
FT STRAND 140..144
FT /evidence="ECO:0007829|PDB:2OML"
FT STRAND 165..173
FT /evidence="ECO:0007829|PDB:2OML"
FT HELIX 179..186
FT /evidence="ECO:0007829|PDB:2OML"
FT STRAND 191..199
FT /evidence="ECO:0007829|PDB:2OML"
FT STRAND 212..214
FT /evidence="ECO:0007829|PDB:2OML"
SQ SEQUENCE 217 AA; 24880 MW; F7C7A7CEDC5FD3F6 CRC64;
MRQFIISENT MQKTSFRNHQ VKRFSSQRST RRKPENQPTR VILFNKPYDV LPQFTDEAGR
KTLKEFIPVQ GVYAAGRLDR DSEGLLVLTN NGALQARLTQ PGKRTGKIYY VQVEGIPTQD
ALEALRNGVT LNDGPTLPAG AELVDEPAWL WPRNPPIRER KSIPTSWLKI TLYEGRNRQV
RRMTAHVGFP TLRLIRYAMG DYSLDNLANG EWREVTD