RLUE_HAEIN
ID RLUE_HAEIN Reviewed; 240 AA.
AC P44827;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase E;
DE EC=5.4.99.20;
DE AltName: Full=rRNA pseudouridylate synthase E;
DE AltName: Full=rRNA-uridine isomerase E;
GN Name=rluE; OrderedLocusNames=HI_0694;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2457
CC in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(2457) in 23S rRNA = pseudouridine(2457) in 23S rRNA;
CC Xref=Rhea:RHEA:38871, Rhea:RHEA-COMP:10091, Rhea:RHEA-COMP:10092,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.20;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
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DR EMBL; L42023; AAC22354.1; -; Genomic_DNA.
DR PIR; I64156; I64156.
DR RefSeq; NP_438854.1; NC_000907.1.
DR AlphaFoldDB; P44827; -.
DR SMR; P44827; -.
DR STRING; 71421.HI_0694; -.
DR EnsemblBacteria; AAC22354; AAC22354; HI_0694.
DR KEGG; hin:HI_0694; -.
DR PATRIC; fig|71421.8.peg.726; -.
DR eggNOG; COG1187; Bacteria.
DR HOGENOM; CLU_024979_8_0_6; -.
DR OMA; HPRTYWV; -.
DR PhylomeDB; P44827; -.
DR BioCyc; HINF71421:G1GJ1-729-MON; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.1560; -; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; rRNA processing.
FT CHAIN 1..240
FT /note="Ribosomal large subunit pseudouridine synthase E"
FT /id="PRO_0000100004"
FT ACT_SITE 91
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 240 AA; 27464 MW; 0AD99EC61F52C01C CRC64;
MMLQAFQNRN HKLMKKNFSA GKLPSKGKSA VNFHRTFKPK LKPKTLSLDE TKVVLFNKPF
DVLTQFTDEQ GRATLKDFIS IPNVYAAGRL DRDSEGLLIL TNNGELQHRL ADPKFKTEKT
YWVQVEGIPE ETDLAQLRKG VELKDGVTKS AKVRLISEPN LWERNPPIRE RKNIPTSWLE
IKISEGRNRQ VRRMTAHIGF PTLRLVRVSM GLLSINGLEN GSFRLLSLDE IKALFQTVKL
