ID   RLUE_PASMU              Reviewed;         241 AA.
AC   Q9CKK7;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase E;
DE            EC=5.4.99.20;
DE   AltName: Full=rRNA pseudouridylate synthase E;
DE   AltName: Full=rRNA-uridine isomerase E;
GN   Name=rluE; OrderedLocusNames=PM1605;
OS   Pasteurella multocida (strain Pm70).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Pasteurella.
OX   NCBI_TaxID=272843;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pm70;
RX   PubMed=11248100; DOI=10.1073/pnas.051634598;
RA   May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.;
RT   "Complete genomic sequence of Pasteurella multocida Pm70.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2457
CC       in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2457) in 23S rRNA = pseudouridine(2457) in 23S rRNA;
CC         Xref=Rhea:RHEA:38871, Rhea:RHEA-COMP:10091, Rhea:RHEA-COMP:10092,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.20;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
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DR   EMBL; AE004439; AAK03689.1; -; Genomic_DNA.
DR   RefSeq; WP_010907249.1; NC_002663.1.
DR   AlphaFoldDB; Q9CKK7; -.
DR   SMR; Q9CKK7; -.
DR   STRING; 747.DR93_454; -.
DR   EnsemblBacteria; AAK03689; AAK03689; PM1605.
DR   KEGG; pmu:PM1605; -.
DR   PATRIC; fig|272843.6.peg.1624; -.
DR   HOGENOM; CLU_024979_8_0_6; -.
DR   OMA; HPRTYWV; -.
DR   Proteomes; UP000000809; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; rRNA processing.
FT   CHAIN           1..241
FT                   /note="Ribosomal large subunit pseudouridine synthase E"
FT                   /id="PRO_0000100005"
FT   REGION          1..34
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..16
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        89
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   241 AA;  28058 MW;  E83F83C83CE66D54 CRC64;
     MKPNTKFNQK TKPRPSSLRS VNKRKAAEIK PQKRPLSFRQ KKTVNFAETQ VILFNKPYDV
     LTQFSDEEGR ATLKAFIPIP HVYPAGRLDR DSEGLVLLTN NGEIQHRLAV PKFNTEKTYF
     AQVEGIPQET DLAQLRQGVV LNDGKTLPAK VRLVPEPNWL WQRNPPIRER KQIPTSWLEI
     KIHEGRNRQV RRMTAHIGFP TLRLIRYQMA LFNLANLEQG QYRQLSEVEL TALYQQLKLK
     K