RLUE_PSEAE
ID RLUE_PSEAE Reviewed; 189 AA.
AC Q9HX48;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase E;
DE EC=5.4.99.20;
DE AltName: Full=rRNA pseudouridylate synthase E;
DE AltName: Full=rRNA-uridine isomerase E;
GN Name=rluE; OrderedLocusNames=PA3968;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2457
CC in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(2457) in 23S rRNA = pseudouridine(2457) in 23S rRNA;
CC Xref=Rhea:RHEA:38871, Rhea:RHEA-COMP:10091, Rhea:RHEA-COMP:10092,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.20;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
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DR EMBL; AE004091; AAG07355.1; -; Genomic_DNA.
DR PIR; A83151; A83151.
DR RefSeq; NP_252657.1; NC_002516.2.
DR RefSeq; WP_003114324.1; NZ_QZGE01000001.1.
DR AlphaFoldDB; Q9HX48; -.
DR SMR; Q9HX48; -.
DR STRING; 287.DR97_3898; -.
DR PaxDb; Q9HX48; -.
DR PRIDE; Q9HX48; -.
DR EnsemblBacteria; AAG07355; AAG07355; PA3968.
DR GeneID; 878864; -.
DR KEGG; pae:PA3968; -.
DR PATRIC; fig|208964.12.peg.4158; -.
DR PseudoCAP; PA3968; -.
DR HOGENOM; CLU_024979_8_1_6; -.
DR InParanoid; Q9HX48; -.
DR OMA; HPRTYWV; -.
DR PhylomeDB; Q9HX48; -.
DR BioCyc; PAER208964:G1FZ6-4041-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.1560; -; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; rRNA processing.
FT CHAIN 1..189
FT /note="Ribosomal large subunit pseudouridine synthase E"
FT /id="PRO_0000100006"
FT ACT_SITE 49
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 189 AA; 21576 MW; E546A1DB0FB6B53E CRC64;
MAKAPPSEPK LLLFNKPFDV LTQFNDEQGR STLKDFIPVP GVYPAGRLDR DSEGLLLLTN
DGRLQARIAD PRHKLPKTYW VQVEGTPDEE QLRRLREGVT LNDGPTLPAE ARLLDEPTLW
ERVPPVRFRK SVPTHWLELV IREGRNRQVR RMTAAVGLPT LRLVRVRIGP WSLDGLGLGE
WREVPARLD
