RLUE_SHIFL
ID RLUE_SHIFL Reviewed; 217 AA.
AC Q83LF6;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 15-AUG-2003, sequence version 2.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase E;
DE EC=5.4.99.20;
DE AltName: Full=rRNA pseudouridylate synthase E;
DE AltName: Full=rRNA-uridine isomerase E;
GN Name=rluE; OrderedLocusNames=SF1154, S1237;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2457
CC in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(2457) in 23S rRNA = pseudouridine(2457) in 23S rRNA;
CC Xref=Rhea:RHEA:38871, Rhea:RHEA-COMP:10091, Rhea:RHEA-COMP:10092,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.20;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN42771.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAP16660.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE005674; AAN42771.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE014073; AAP16660.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_707064.1; NC_004337.2.
DR RefSeq; WP_001248670.1; NZ_WPGW01000001.1.
DR AlphaFoldDB; Q83LF6; -.
DR SMR; Q83LF6; -.
DR STRING; 198214.SF1154; -.
DR EnsemblBacteria; AAN42771; AAN42771; SF1154.
DR EnsemblBacteria; AAP16660; AAP16660; S1237.
DR GeneID; 1024083; -.
DR GeneID; 58390815; -.
DR KEGG; sfl:SF1154; -.
DR KEGG; sfx:S1237; -.
DR PATRIC; fig|198214.7.peg.1355; -.
DR HOGENOM; CLU_024979_8_0_6; -.
DR OrthoDB; 1037615at2; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.1560; -; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; rRNA processing.
FT CHAIN 1..217
FT /note="Ribosomal large subunit pseudouridine synthase E"
FT /id="PRO_0000100009"
FT ACT_SITE 79
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 217 AA; 24950 MW; 480FE69B34427D2E CRC64;
MRQFIISENT MQKTSFRNHQ VKRFSSQRST RRKHENQPTR VILFNKPYDV LPQFTDETGR
KTLKEFIPVQ GVYAAGRLDR DSEGLLVLTN NGALQARLTQ PGKRTGKIYY VQVEGIPTQD
ALEALRNGVT LNDGPTLPAG AELVDEPAWL WPRNPPIRER KSIPTSWLKI TLYEGRNRQV
RRMTAHVGFP TLRLIRYAMG DYSLDNLANG EWREVTD
