ID   RLUE_VIBCH              Reviewed;         241 AA.
AC   Q9F855; Q9KSW6;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   15-AUG-2003, sequence version 2.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase E;
DE            EC=5.4.99.20;
DE   AltName: Full=rRNA pseudouridylate synthase E;
DE   AltName: Full=rRNA-uridine isomerase E;
GN   Name=rluE; OrderedLocusNames=VC_1140;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 25870 / Classical Inaba 569B / Serotype O1;
RA   Haralalka S., Roychoudhury S., Chaudhuri K.;
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2457
CC       in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2457) in 23S rRNA = pseudouridine(2457) in 23S rRNA;
CC         Xref=Rhea:RHEA:38871, Rhea:RHEA-COMP:10091, Rhea:RHEA-COMP:10092,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.20;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF94299.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF261151; AAG16128.1; -; Genomic_DNA.
DR   EMBL; AE003852; AAF94299.1; ALT_INIT; Genomic_DNA.
DR   PIR; A82236; A82236.
DR   RefSeq; NP_230785.2; NC_002505.1.
DR   RefSeq; WP_000098720.1; NZ_LT906614.1.
DR   AlphaFoldDB; Q9F855; -.
DR   SMR; Q9F855; -.
DR   STRING; 243277.VC_1140; -.
DR   DNASU; 2614410; -.
DR   EnsemblBacteria; AAF94299; AAF94299; VC_1140.
DR   GeneID; 57739816; -.
DR   KEGG; vch:VC_1140; -.
DR   PATRIC; fig|243277.26.peg.1089; -.
DR   eggNOG; COG1187; Bacteria.
DR   HOGENOM; CLU_024979_8_0_6; -.
DR   OMA; HPRTYWV; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; rRNA processing.
FT   CHAIN           1..241
FT                   /note="Ribosomal large subunit pseudouridine synthase E"
FT                   /id="PRO_0000100010"
FT   REGION          1..65
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        104
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        97
FT                   /note="V -> G (in Ref. 1; AAG16128)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   241 AA;  27007 MW;  BC50548579111BF3 CRC64;
     MSSRLSSDAT SSSSDQRKSH FKQRAAKNAG HPPTRANRKS VANKKKNATQ TALSPKRPLS
     PAERKVILFN KPYDTLSQFT DGDGRKTLAD YIPIKDVYAA GRLDRDSEGL LILTNDGILQ
     ARLTQPQSKA PKTYWVQVEG SPQESDLEAL RHGVTLKDGP TLPAKVDIMP EPTLWPRNPP
     VRFRAAIPTT WLAITLMEGR NRQVRRMTAH IGFPTLRLIR YSMGDWNLGD LQPGEWREVT
     L