ID   RLUE_VIBPA              Reviewed;         238 AA.
AC   Q87QY8;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase E;
DE            EC=5.4.99.20;
DE   AltName: Full=rRNA pseudouridylate synthase E;
DE   AltName: Full=rRNA-uridine isomerase E;
GN   Name=rluE; OrderedLocusNames=VP1010;
OS   Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=223926;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RIMD 2210633;
RX   PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA   Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA   Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA   Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT   "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT   distinct from that of V. cholerae.";
RL   Lancet 361:743-749(2003).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2457
CC       in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2457) in 23S rRNA = pseudouridine(2457) in 23S rRNA;
CC         Xref=Rhea:RHEA:38871, Rhea:RHEA-COMP:10091, Rhea:RHEA-COMP:10092,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.20;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BA000031; BAC59273.1; -; Genomic_DNA.
DR   RefSeq; NP_797389.1; NC_004603.1.
DR   RefSeq; WP_005481826.1; NC_004603.1.
DR   AlphaFoldDB; Q87QY8; -.
DR   SMR; Q87QY8; -.
DR   STRING; 223926.28805997; -.
DR   EnsemblBacteria; BAC59273; BAC59273; BAC59273.
DR   GeneID; 1188514; -.
DR   KEGG; vpa:VP1010; -.
DR   PATRIC; fig|223926.6.peg.957; -.
DR   eggNOG; COG1187; Bacteria.
DR   HOGENOM; CLU_024979_8_0_6; -.
DR   OMA; HPRTYWV; -.
DR   Proteomes; UP000002493; Chromosome 1.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; rRNA processing.
FT   CHAIN           1..238
FT                   /note="Ribosomal large subunit pseudouridine synthase E"
FT                   /id="PRO_0000100011"
FT   REGION          1..54
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..15
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        24..39
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        101
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   238 AA;  27025 MW;  C454D197D1CA6837 CRC64;
     MSSRTGHEKG RRTSQSKTGH FKRSGKPNRV EDQTTRRHPT RTAKAKAKAK ANKPRVDLQN
     RKVIIFNKPY DTLSQFTDGD GRKTLADYIP VKDVYAAGRL DRDSEGLMVL TNDGILQAKL
     TQPKSKSPKT YWVQVDGAPQ EQDLEKLRKG VELKDGMTLP AKVEVIDAPT IWERNPPVRF
     RANIPTTWLA ITIIEGRNRQ VRRMTAHIGF PTLRLVRYSM GDITLGDLQP GQWKEIQL