RLUE_XANAC
ID RLUE_XANAC Reviewed; 190 AA.
AC Q8PQN3;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 15-AUG-2003, sequence version 2.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase E;
DE EC=5.4.99.20;
DE AltName: Full=rRNA pseudouridylate synthase E;
DE AltName: Full=rRNA-uridine isomerase E;
GN Name=rluE; OrderedLocusNames=XAC0290;
OS Xanthomonas axonopodis pv. citri (strain 306).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Xanthomonas.
OX NCBI_TaxID=190486;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=306;
RX PubMed=12024217; DOI=10.1038/417459a;
RA da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R.,
RA Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr.,
RA Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G.,
RA Cannavan F., Cardozo J., Chambergo F., Ciapina L.P., Cicarelli R.M.B.,
RA Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B.,
RA Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F.,
RA Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T.,
RA Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A.,
RA Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J.,
RA Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M.,
RA Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A.,
RA Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A.,
RA Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M.,
RA Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P.;
RT "Comparison of the genomes of two Xanthomonas pathogens with differing host
RT specificities.";
RL Nature 417:459-463(2002).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2457
CC in 23S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(2457) in 23S rRNA = pseudouridine(2457) in 23S rRNA;
CC Xref=Rhea:RHEA:38871, Rhea:RHEA-COMP:10091, Rhea:RHEA-COMP:10092,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.20;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM35182.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE008923; AAM35182.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_015462765.1; NC_003919.1.
DR AlphaFoldDB; Q8PQN3; -.
DR SMR; Q8PQN3; -.
DR STRING; 190486.XAC0290; -.
DR EnsemblBacteria; AAM35182; AAM35182; XAC0290.
DR GeneID; 66909503; -.
DR KEGG; xac:XAC0290; -.
DR eggNOG; COG1187; Bacteria.
DR HOGENOM; CLU_024979_8_1_6; -.
DR OMA; HPRTYWV; -.
DR Proteomes; UP000000576; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.1560; -; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
PE 3: Inferred from homology;
KW Isomerase; rRNA processing.
FT CHAIN 1..190
FT /note="Ribosomal large subunit pseudouridine synthase E"
FT /id="PRO_0000100013"
FT ACT_SITE 42
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 190 AA; 21304 MW; 775F55B9F290AE50 CRC64;
MLVLLNKPYG VLSQFSDRSQ PPKRTLAEFG LPPDVYAAGR LDHDSEGLLV LTDDGALAHR
LTDPRHKQPK TYWVQVEGAP QAEHLQALRD GVALNDGPTR PAQVRMLDPA PQLWPRDPPV
RVRKTVPDAW LELQITEGRN RQVRRMTAAV GLPTLRLVRV AIGDWRLDAL APGQWRAEAT
TNARPSRSRR