ID   RLUE_XANCP              Reviewed;         187 AA.
AC   Q8PDR2;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase E;
DE            EC=5.4.99.20;
DE   AltName: Full=rRNA pseudouridylate synthase E;
DE   AltName: Full=rRNA-uridine isomerase E;
GN   Name=rluE; OrderedLocusNames=XCC0273;
OS   Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB
OS   528 / LMG 568 / P 25).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Xanthomonas.
OX   NCBI_TaxID=190485;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25;
RX   PubMed=12024217; DOI=10.1038/417459a;
RA   da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R.,
RA   Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr.,
RA   Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G.,
RA   Cannavan F., Cardozo J., Chambergo F., Ciapina L.P., Cicarelli R.M.B.,
RA   Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B.,
RA   Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F.,
RA   Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T.,
RA   Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A.,
RA   Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J.,
RA   Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M.,
RA   Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A.,
RA   Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A.,
RA   Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M.,
RA   Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P.;
RT   "Comparison of the genomes of two Xanthomonas pathogens with differing host
RT   specificities.";
RL   Nature 417:459-463(2002).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-2457
CC       in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2457) in 23S rRNA = pseudouridine(2457) in 23S rRNA;
CC         Xref=Rhea:RHEA:38871, Rhea:RHEA-COMP:10091, Rhea:RHEA-COMP:10092,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.20;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE008922; AAM39592.1; -; Genomic_DNA.
DR   RefSeq; NP_635668.1; NC_003902.1.
DR   RefSeq; WP_011035528.1; NC_003902.1.
DR   AlphaFoldDB; Q8PDR2; -.
DR   SMR; Q8PDR2; -.
DR   STRING; 340.xcc-b100_0298; -.
DR   EnsemblBacteria; AAM39592; AAM39592; XCC0273.
DR   KEGG; xcc:XCC0273; -.
DR   PATRIC; fig|190485.4.peg.303; -.
DR   eggNOG; COG1187; Bacteria.
DR   HOGENOM; CLU_024979_8_1_6; -.
DR   OMA; HPRTYWV; -.
DR   Proteomes; UP000001010; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; rRNA processing.
FT   CHAIN           1..187
FT                   /note="Ribosomal large subunit pseudouridine synthase E"
FT                   /id="PRO_0000100014"
FT   ACT_SITE        42
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   187 AA;  20904 MW;  6584365AE6C730C3 CRC64;
     MLVLLNKPYG VLSQFSDRST PPKRTLAEFG LPPQVYAAGR LDHDSEGLLL LTDDGPLAHR
     LTDPRHKQPK TYWVQVEGDP DTSQLQALCD GVLLNDGPTR PANVRRLECA PTLWPRDPPV
     RVRKTVPDAW LEVQITEGRN RQVRRMTASV GLPTLRLVRV AIGAWSLASL QPGQWRVDDA
     RAVRPAR