RM08_YEAST
ID RM08_YEAST Reviewed; 238 AA.
AC P22353; D6VWB8;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 4.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=54S ribosomal protein L8, mitochondrial;
DE AltName: Full=Mitochondrial large ribosomal subunit protein bL17m {ECO:0000303|PubMed:24675956};
DE AltName: Full=YmL8;
GN Name=MRPL8; OrderedLocusNames=YJL063C; ORFNames=HRD238, J1125;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-28.
RC STRAIN=07173;
RX PubMed=2183197; DOI=10.1093/nar/18.6.1521;
RA Kitakawa M., Grohmann L., Graack H.-R., Isono K.;
RT "Cloning and characterization of nuclear genes for two mitochondrial
RT ribosomal proteins in Saccharomyces cerevisiae.";
RL Nucleic Acids Res. 18:1521-1529(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7762302; DOI=10.1002/yea.320110108;
RA Vandenbol M., Durand P., Dion C., Portetelle D., Hilger F.;
RT "Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces
RT cerevisiae includes the mitochondrial ribosomal protein L8.";
RL Yeast 11:57-60(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP TRANSPORT INTO MITOCHONDRIA.
RX PubMed=8513807; DOI=10.1111/j.1432-1033.1993.tb17956.x;
RA Matsushita Y., Isono K.;
RT "Mitochondrial transport of mitoribosomal proteins, YmL8 and YmL20, in
RT Saccharomyces cerevisiae.";
RL Eur. J. Biochem. 214:577-585(1993).
RN [7]
RP IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=12392552; DOI=10.1046/j.1432-1033.2002.03226.x;
RA Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.;
RT "Tag-mediated isolation of yeast mitochondrial ribosome and mass
RT spectrometric identification of its new components.";
RL Eur. J. Biochem. 269:5203-5214(2002).
RN [8]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [9]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [10]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 76625 / YPH499;
RX PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E.,
RA Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P.,
RA Pfanner N., Meisinger C.;
RT "The proteome of Saccharomyces cerevisiae mitochondria.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
RN [11]
RP SUBCELLULAR LOCATION.
RX PubMed=25609543; DOI=10.1038/ncomms7019;
RA Pfeffer S., Woellhaf M.W., Herrmann J.M., Forster F.;
RT "Organization of the mitochondrial translation machinery studied in situ by
RT cryoelectron tomography.";
RL Nat. Commun. 6:6019-6019(2015).
RN [12]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.20 ANGSTROMS), AND SUBUNIT.
RX PubMed=24675956; DOI=10.1126/science.1249410;
RA Amunts A., Brown A., Bai X.C., Llacer J.L., Hussain T., Emsley P., Long F.,
RA Murshudov G., Scheres S.H., Ramakrishnan V.;
RT "Structure of the yeast mitochondrial large ribosomal subunit.";
RL Science 343:1485-1489(2014).
CC -!- FUNCTION: Component of the mitochondrial ribosome (mitoribosome), a
CC dedicated translation machinery responsible for the synthesis of
CC mitochondrial genome-encoded proteins, including at least some of the
CC essential transmembrane subunits of the mitochondrial respiratory
CC chain. The mitoribosomes are attached to the mitochondrial inner
CC membrane and translation products are cotranslationally integrated into
CC the membrane. {ECO:0000305|PubMed:24675956,
CC ECO:0000305|PubMed:25609543}.
CC -!- SUBUNIT: Component of the mitochondrial large ribosomal subunit (mt-
CC LSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S)
CC and a large (54S) subunit. The 37S small subunit contains a 15S
CC ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large
CC subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins.
CC {ECO:0000269|PubMed:12392552, ECO:0000269|PubMed:24675956}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:14576278}. Note=Mitoribosomes are tethered to the
CC mitochondrial inner membrane and spatially aligned with the membrane
CC insertion machinery through two distinct membrane contact sites, formed
CC by the 21S rRNA expansion segment 96-ES1 and the inner membrane protein
CC MBA1. {ECO:0000269|PubMed:25609543}.
CC -!- MISCELLANEOUS: Although this protein has evidently no mitochondrial
CC targeting pre-sequence, it is efficiently transported into
CC mitochondria.
CC -!- MISCELLANEOUS: Present with 3000 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bL17 family.
CC {ECO:0000305}.
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DR EMBL; X53841; CAA37834.1; -; Genomic_DNA.
DR EMBL; Z34288; CAA84060.1; -; Genomic_DNA.
DR EMBL; Z49338; CAA89354.1; -; Genomic_DNA.
DR EMBL; AY558249; AAS56575.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08734.1; -; Genomic_DNA.
DR PIR; S50809; S50809.
DR RefSeq; NP_012472.1; NM_001181496.1.
DR PDB; 3J6B; EM; 3.20 A; L=1-238.
DR PDB; 5MRC; EM; 3.25 A; L=2-238.
DR PDB; 5MRE; EM; 3.75 A; L=2-238.
DR PDB; 5MRF; EM; 4.97 A; L=2-238.
DR PDBsum; 3J6B; -.
DR PDBsum; 5MRC; -.
DR PDBsum; 5MRE; -.
DR PDBsum; 5MRF; -.
DR AlphaFoldDB; P22353; -.
DR SMR; P22353; -.
DR BioGRID; 33690; 116.
DR ComplexPortal; CPX-1602; 54S mitochondrial large ribosomal subunit.
DR DIP; DIP-5672N; -.
DR IntAct; P22353; 9.
DR MINT; P22353; -.
DR STRING; 4932.YJL063C; -.
DR MaxQB; P22353; -.
DR PaxDb; P22353; -.
DR PRIDE; P22353; -.
DR EnsemblFungi; YJL063C_mRNA; YJL063C; YJL063C.
DR GeneID; 853382; -.
DR KEGG; sce:YJL063C; -.
DR SGD; S000003599; MRPL8.
DR VEuPathDB; FungiDB:YJL063C; -.
DR eggNOG; KOG3280; Eukaryota.
DR GeneTree; ENSGT00390000010698; -.
DR HOGENOM; CLU_074407_1_3_1; -.
DR InParanoid; P22353; -.
DR OMA; RDMRFAM; -.
DR BioCyc; YEAST:G3O-31525-MON; -.
DR PRO; PR:P22353; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P22353; protein.
DR GO; GO:0015934; C:large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0005743; C:mitochondrial inner membrane; IC:ComplexPortal.
DR GO; GO:0005762; C:mitochondrial large ribosomal subunit; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0003735; F:structural constituent of ribosome; IDA:SGD.
DR GO; GO:0032543; P:mitochondrial translation; IC:SGD.
DR Gene3D; 3.90.1030.10; -; 1.
DR InterPro; IPR040894; Mrpl_C.
DR InterPro; IPR000456; Ribosomal_L17.
DR InterPro; IPR036373; Ribosomal_L17_sf.
DR PANTHER; PTHR14413; PTHR14413; 1.
DR Pfam; PF18502; Mrpl_C; 1.
DR Pfam; PF01196; Ribosomal_L17; 1.
DR SUPFAM; SSF64263; SSF64263; 1.
DR TIGRFAMs; TIGR00059; L17; 1.
DR PROSITE; PS01167; RIBOSOMAL_L17; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Mitochondrion; Reference proteome;
KW Ribonucleoprotein; Ribosomal protein.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:2183197"
FT CHAIN 2..238
FT /note="54S ribosomal protein L8, mitochondrial"
FT /id="PRO_0000175550"
FT CONFLICT 2
FT /note="T -> G (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 82
FT /note="D -> G (in Ref. 1; CAA37834)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 238 AA; 26945 MW; A130EFD95E8719BA CRC64;
MTVGIARKLS RDKAHRDALL KNLACQLFQH ESIVSTHAKC KEASRVAERI ITWTKRAITT
SNSVAQAELK SQIQSQLFLA GDNRKLMKRL FSEIAPRYLE RPGGYTRVLR LEPRANDSAP
QSVLELVDSP VMSESHTVNR GNLKMWLLVK SVINDDANQL PHNPLTLQNL HKVAKFKAEA
QLHGEIMLIK QVLLKEMSLP YDEALENERT QALLKEVYSS SLPKKTKKPS SYVMVPRP
