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RM31_YEAST
ID   RM31_YEAST              Reviewed;         131 AA.
AC   P14063; D6VX59;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   03-AUG-2022, entry version 163.
DE   RecName: Full=54S ribosomal protein L31, mitochondrial;
DE   AltName: Full=Mitochondrial large ribosomal subunit protein mL60 {ECO:0000303|PubMed:24675956};
DE   AltName: Full=YmL31;
DE   Flags: Precursor;
GN   Name=MRPL31; OrderedLocusNames=YKL138C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=07173;
RX   PubMed=2666132; DOI=10.1111/j.1432-1033.1989.tb14907.x;
RA   Grohmann L., Graack H.-R., Kitakawa M.;
RT   "Molecular cloning of the nuclear gene for mitochondrial ribosomal protein
RT   YmL31 from Saccharomyces cerevisiae.";
RL   Eur. J. Biochem. 183:155-160(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8196765; DOI=10.1038/369371a0;
RA   Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA   Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA   Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA   Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA   Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA   Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA   Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA   Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA   Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA   Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA   Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA   Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA   Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA   Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA   Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA   Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA   Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA   Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA   Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA   van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA   von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA   Becker I., Mewes H.-W.;
RT   "Complete DNA sequence of yeast chromosome XI.";
RL   Nature 369:371-378(1994).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   PROTEIN SEQUENCE OF 13-50, AND SUBUNIT.
RC   STRAIN=07173;
RX   PubMed=3060376; DOI=10.1016/0014-5793(88)80975-x;
RA   Graack H.-R., Grohmann L., Choli T.;
RT   "Mitochondrial ribosomes of yeast: isolation of individual proteins and N-
RT   terminal sequencing.";
RL   FEBS Lett. 242:4-8(1988).
RN   [5]
RP   IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=12392552; DOI=10.1046/j.1432-1033.2002.03226.x;
RA   Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.;
RT   "Tag-mediated isolation of yeast mitochondrial ribosome and mass
RT   spectrometric identification of its new components.";
RL   Eur. J. Biochem. 269:5203-5214(2002).
RN   [6]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [7]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [8]
RP   SUBCELLULAR LOCATION.
RX   PubMed=25609543; DOI=10.1038/ncomms7019;
RA   Pfeffer S., Woellhaf M.W., Herrmann J.M., Forster F.;
RT   "Organization of the mitochondrial translation machinery studied in situ by
RT   cryoelectron tomography.";
RL   Nat. Commun. 6:6019-6019(2015).
RN   [9]
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.20 ANGSTROMS), AND SUBUNIT.
RX   PubMed=24675956; DOI=10.1126/science.1249410;
RA   Amunts A., Brown A., Bai X.C., Llacer J.L., Hussain T., Emsley P., Long F.,
RA   Murshudov G., Scheres S.H., Ramakrishnan V.;
RT   "Structure of the yeast mitochondrial large ribosomal subunit.";
RL   Science 343:1485-1489(2014).
CC   -!- FUNCTION: Component of the mitochondrial ribosome (mitoribosome), a
CC       dedicated translation machinery responsible for the synthesis of
CC       mitochondrial genome-encoded proteins, including at least some of the
CC       essential transmembrane subunits of the mitochondrial respiratory
CC       chain. The mitoribosomes are attached to the mitochondrial inner
CC       membrane and translation products are cotranslationally integrated into
CC       the membrane. {ECO:0000305|PubMed:24675956,
CC       ECO:0000305|PubMed:25609543}.
CC   -!- SUBUNIT: Component of the mitochondrial large ribosomal subunit (mt-
CC       LSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S)
CC       and a large (54S) subunit. The 37S small subunit contains a 15S
CC       ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large
CC       subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins.
CC       {ECO:0000269|PubMed:12392552, ECO:0000269|PubMed:24675956,
CC       ECO:0000269|PubMed:3060376}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095}.
CC       Note=Mitoribosomes are tethered to the mitochondrial inner membrane and
CC       spatially aligned with the membrane insertion machinery through two
CC       distinct membrane contact sites, formed by the 21S rRNA expansion
CC       segment 96-ES1 and the inner membrane protein MBA1.
CC       {ECO:0000269|PubMed:25609543}.
CC   -!- MISCELLANEOUS: Present with 2100 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the mitochondrion-specific ribosomal protein
CC       mL60 family. {ECO:0000305}.
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DR   EMBL; X15099; CAA33202.1; -; Genomic_DNA.
DR   EMBL; Z28138; CAA81979.1; -; Genomic_DNA.
DR   EMBL; BK006944; DAA09025.1; -; Genomic_DNA.
DR   PIR; S22245; R5BY31.
DR   RefSeq; NP_012784.1; NM_001179704.1.
DR   PDB; 3J6B; EM; 3.20 A; c=1-131.
DR   PDB; 5MRC; EM; 3.25 A; c=13-131.
DR   PDB; 5MRE; EM; 3.75 A; c=13-131.
DR   PDB; 5MRF; EM; 4.97 A; c=13-131.
DR   PDBsum; 3J6B; -.
DR   PDBsum; 5MRC; -.
DR   PDBsum; 5MRE; -.
DR   PDBsum; 5MRF; -.
DR   AlphaFoldDB; P14063; -.
DR   SMR; P14063; -.
DR   BioGRID; 33999; 32.
DR   ComplexPortal; CPX-1602; 54S mitochondrial large ribosomal subunit.
DR   DIP; DIP-5089N; -.
DR   IntAct; P14063; 3.
DR   STRING; 4932.YKL138C; -.
DR   MaxQB; P14063; -.
DR   PaxDb; P14063; -.
DR   PRIDE; P14063; -.
DR   EnsemblFungi; YKL138C_mRNA; YKL138C; YKL138C.
DR   GeneID; 853720; -.
DR   KEGG; sce:YKL138C; -.
DR   SGD; S000001621; MRPL31.
DR   VEuPathDB; FungiDB:YKL138C; -.
DR   eggNOG; ENOG502S81F; Eukaryota.
DR   HOGENOM; CLU_141719_0_0_1; -.
DR   InParanoid; P14063; -.
DR   OMA; KYTVFDR; -.
DR   BioCyc; YEAST:G3O-31915-MON; -.
DR   PRO; PR:P14063; -.
DR   Proteomes; UP000002311; Chromosome XI.
DR   RNAct; P14063; protein.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IC:ComplexPortal.
DR   GO; GO:0005762; C:mitochondrial large ribosomal subunit; IDA:SGD.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR   GO; GO:0003735; F:structural constituent of ribosome; IDA:SGD.
DR   GO; GO:0032543; P:mitochondrial translation; IC:SGD.
DR   InterPro; IPR016340; Ribosomal_L31_mit.
DR   PANTHER; PTHR28271; PTHR28271; 1.
DR   Pfam; PF09784; L31; 1.
DR   PIRSF; PIRSF002216; MRPL31_prd; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Mitochondrion; Reference proteome;
KW   Ribonucleoprotein; Ribosomal protein; Transit peptide.
FT   TRANSIT         1..12
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000269|PubMed:2666132,
FT                   ECO:0000269|PubMed:3060376"
FT   CHAIN           13..131
FT                   /note="54S ribosomal protein L31, mitochondrial"
FT                   /id="PRO_0000030579"
SQ   SEQUENCE   131 AA;  15520 MW;  A4B3C3041054519A CRC64;
     MFGPFKLTSP VAGGLLWKIP WRMSTHQKTR QRERLRNVDQ VIKQLTLGLH VQRCQDKGLT
     YQEAMESKKK YKPRSKSLRL LNKPSVFPKE NQMSSKDKYW TFDKKAVGYR KGIHKVPKWT
     KISIRKAPKF F
 
 
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