RM32_MOUSE
ID RM32_MOUSE Reviewed; 187 AA.
AC Q9DCI9; Q91XR6;
DT 26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=39S ribosomal protein L32, mitochondrial;
DE Short=L32mt;
DE Short=MRP-L32;
DE AltName: Full=Heart-expressed gene 1 protein;
DE Flags: Precursor;
GN Name=Mrpl32; Synonyms=Heg1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11279069; DOI=10.1074/jbc.m100432200;
RA Suzuki T., Terasaki M., Takemoto-Hori C., Hanada T., Ueda T., Wada A.,
RA Watanabe K.;
RT "Structural compensation for the deficit of rRNA with proteins in the
RT mammalian mitochondrial ribosome. Systematic analysis of protein components
RT of the large ribosomal subunit from mammalian mitochondria.";
RL J. Biol. Chem. 276:21724-21736(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Heart;
RA Schepler A., Arnemann J.;
RT "HEG1 (heart expressed gene) - a new marker gene for heart development in
RT the mouse.";
RL Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Kidney, and Liver;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBUNIT: Component of the mitochondrial ribosome large subunit (39S)
CC which comprises a 16S rRNA and about 50 distinct proteins.
CC {ECO:0000250|UniProtKB:Q9BYC8}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q9BYC8}.
CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bL32 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK69482.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB049650; BAB40855.1; -; mRNA.
DR EMBL; AK002753; BAB22330.1; -; mRNA.
DR EMBL; AF279255; AAK69482.1; ALT_INIT; mRNA.
DR EMBL; BC052214; AAH52214.1; -; mRNA.
DR CCDS; CCDS26249.1; -.
DR RefSeq; NP_083547.1; NM_029271.2.
DR AlphaFoldDB; Q9DCI9; -.
DR SMR; Q9DCI9; -.
DR BioGRID; 217452; 31.
DR ComplexPortal; CPX-5302; 39S mitochondrial large ribosomal subunit.
DR STRING; 10090.ENSMUSP00000015816; -.
DR PhosphoSitePlus; Q9DCI9; -.
DR EPD; Q9DCI9; -.
DR MaxQB; Q9DCI9; -.
DR PaxDb; Q9DCI9; -.
DR PeptideAtlas; Q9DCI9; -.
DR PRIDE; Q9DCI9; -.
DR ProteomicsDB; 299830; -.
DR Antibodypedia; 57638; 92 antibodies from 19 providers.
DR DNASU; 75398; -.
DR Ensembl; ENSMUST00000220621; ENSMUSP00000152345; ENSMUSG00000015672.
DR GeneID; 75398; -.
DR KEGG; mmu:75398; -.
DR UCSC; uc007pnj.1; mouse.
DR CTD; 64983; -.
DR MGI; MGI:2137226; Mrpl32.
DR VEuPathDB; HostDB:ENSMUSG00000015672; -.
DR eggNOG; KOG4080; Eukaryota.
DR GeneTree; ENSGT00390000014996; -.
DR HOGENOM; CLU_116455_1_0_1; -.
DR InParanoid; Q9DCI9; -.
DR OMA; HCYDKVR; -.
DR OrthoDB; 1600646at2759; -.
DR PhylomeDB; Q9DCI9; -.
DR TreeFam; TF106139; -.
DR Reactome; R-MMU-5389840; Mitochondrial translation elongation.
DR Reactome; R-MMU-5419276; Mitochondrial translation termination.
DR BioGRID-ORCS; 75398; 18 hits in 73 CRISPR screens.
DR ChiTaRS; Mrpl32; mouse.
DR PRO; PR:Q9DCI9; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q9DCI9; protein.
DR Bgee; ENSMUSG00000015672; Expressed in interventricular septum and 256 other tissues.
DR ExpressionAtlas; Q9DCI9; baseline and differential.
DR Genevisible; Q9DCI9; MM.
DR GO; GO:0005829; C:cytosol; IEA:UniProt.
DR GO; GO:0005743; C:mitochondrial inner membrane; IC:ComplexPortal.
DR GO; GO:0005762; C:mitochondrial large ribosomal subunit; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0003735; F:structural constituent of ribosome; ISO:MGI.
DR GO; GO:0032543; P:mitochondrial translation; IC:ComplexPortal.
DR GO; GO:0006412; P:translation; ISO:MGI.
DR InterPro; IPR002677; Ribosomal_L32p.
DR InterPro; IPR011332; Ribosomal_zn-bd.
DR Pfam; PF01783; Ribosomal_L32p; 1.
DR SUPFAM; SSF57829; SSF57829; 1.
PE 1: Evidence at protein level;
KW Mitochondrion; Reference proteome; Ribonucleoprotein; Ribosomal protein;
KW Transit peptide.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..187
FT /note="39S ribosomal protein L32, mitochondrial"
FT /id="PRO_0000030514"
FT CONFLICT 12
FT /note="W -> M (in Ref. 3; AAK69482)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 187 AA; 21733 MW; 1AB03A656D404F2D CRC64;
MAPSLLLLSL PWPVRPGPLQ RCWELLQRQL QQSWSRFVSP PWAPALAVQR PSILTELAHD
TCENKENSSF LDSIFWMAAP KNRRTIEVNR CRRRNPQKLI KIKNNIDICP ECGHLKQKHV
LCGYCYEKVR QETTKIRQQI GAQEGGPFRA PSVETMVLYT GEKPSEKDQG KRIVERNIKR
PSWFTQN
