RM33_HUMAN
ID RM33_HUMAN Reviewed; 65 AA.
AC O75394; Q53RZ6; Q5FVE3; Q96Q50;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=39S ribosomal protein L33, mitochondrial;
DE Short=L33mt;
DE Short=MRP-L33;
DE AltName: Full=Mitochondrial large ribosomal subunit protein bL33m {ECO:0000303|PubMed:25278503};
DE Flags: Precursor;
GN Name=MRPL33; Synonyms=C2orf1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Umbilical cord blood;
RX PubMed=9653160; DOI=10.1073/pnas.95.14.8175;
RA Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H.,
RA He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H.,
RA Wang Y.-X., Chen S.-J., Chen Z.;
RT "Identification of genes expressed in human CD34(+) hematopoietic
RT stem/progenitor cells by expressed sequence tags and efficient full-length
RT cDNA cloning.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P.,
RA Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y.,
RA LaBaer J.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, Pituitary, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 52-65.
RX PubMed=11543634; DOI=10.1006/geno.2001.6622;
RA Kenmochi N., Suzuki T., Uechi T., Magoori M., Kuniba M., Higa S.,
RA Watanabe K., Tanaka T.;
RT "The human mitochondrial ribosomal protein genes: mapping of 54 genes to
RT the chromosomes and implications for human disorders.";
RL Genomics 77:65-70(2001).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
RN [10] {ECO:0007744|PDB:3J7Y}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.40 ANGSTROMS), SUBCELLULAR LOCATION,
RP AND SUBUNIT.
RX PubMed=25278503; DOI=10.1126/science.1258026;
RA Brown A., Amunts A., Bai X.C., Sugimoto Y., Edwards P.C., Murshudov G.,
RA Scheres S.H., Ramakrishnan V.;
RT "Structure of the large ribosomal subunit from human mitochondria.";
RL Science 346:718-722(2014).
RN [11] {ECO:0007744|PDB:3J9M}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS), SUBCELLULAR LOCATION,
RP AND SUBUNIT.
RX PubMed=25838379; DOI=10.1126/science.aaa1193;
RA Amunts A., Brown A., Toots J., Scheres S.H., Ramakrishnan V.;
RT "Ribosome. The structure of the human mitochondrial ribosome.";
RL Science 348:95-98(2015).
RN [12] {ECO:0007744|PDB:5OOL, ECO:0007744|PDB:5OOM}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.03 ANGSTROMS), SUBCELLULAR LOCATION,
RP AND SUBUNIT.
RX PubMed=28892042; DOI=10.1038/nsmb.3464;
RA Brown A., Rathore S., Kimanius D., Aibara S., Bai X.C., Rorbach J.,
RA Amunts A., Ramakrishnan V.;
RT "Structures of the human mitochondrial ribosome in native states of
RT assembly.";
RL Nat. Struct. Mol. Biol. 24:866-869(2017).
CC -!- SUBUNIT: Component of the mitochondrial large ribosomal subunit (mt-
CC LSU) (PubMed:28892042, PubMed:25838379, PubMed:25278503). Mature
CC mammalian 55S mitochondrial ribosomes consist of a small (28S) and a
CC large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA
CC (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains
CC a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA
CC (mt-tRNA(Val)), which plays an integral structural role, and 52
CC different proteins. {ECO:0000269|PubMed:25278503,
CC ECO:0000269|PubMed:25838379, ECO:0000269|PubMed:28892042}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:25278503,
CC ECO:0000269|PubMed:25838379, ECO:0000269|PubMed:28892042}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O75394-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O75394-2; Sequence=VSP_042822;
CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bL33 family.
CC {ECO:0000305}.
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DR EMBL; AF047440; AAC39891.1; -; mRNA.
DR EMBL; CR542117; CAG46914.1; -; mRNA.
DR EMBL; CR456888; CAG33169.1; -; mRNA.
DR EMBL; AC021171; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC074091; AAX93204.1; -; Genomic_DNA.
DR EMBL; AC110084; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471053; EAX00552.1; -; Genomic_DNA.
DR EMBL; BC009475; AAH09475.1; -; mRNA.
DR EMBL; BC020834; AAH20834.1; -; mRNA.
DR EMBL; BC090047; AAH90047.1; -; mRNA.
DR EMBL; AB051623; BAB54951.1; -; Genomic_DNA.
DR CCDS; CCDS1761.1; -. [O75394-1]
DR CCDS; CCDS33167.1; -. [O75394-2]
DR RefSeq; NP_004882.1; NM_004891.3. [O75394-1]
DR RefSeq; NP_663303.1; NM_145330.2. [O75394-2]
DR PDB; 3IY9; EM; 14.10 A; P=9-60.
DR PDB; 3J7Y; EM; 3.40 A; 1=1-65.
DR PDB; 3J9M; EM; 3.50 A; 1=1-65.
DR PDB; 5OOL; EM; 3.06 A; 1=1-65.
DR PDB; 5OOM; EM; 3.03 A; 1=1-65.
DR PDB; 6I9R; EM; 3.90 A; 1=1-65.
DR PDB; 6NU2; EM; 3.90 A; 1=14-65.
DR PDB; 6NU3; EM; 4.40 A; 1=1-65.
DR PDB; 6VLZ; EM; 2.97 A; 1=1-65.
DR PDB; 6VMI; EM; 2.96 A; 1=1-65.
DR PDB; 6ZM5; EM; 2.89 A; 1=1-65.
DR PDB; 6ZM6; EM; 2.59 A; 1=1-65.
DR PDB; 6ZS9; EM; 4.00 A; 1=1-65.
DR PDB; 6ZSA; EM; 4.00 A; 1=1-65.
DR PDB; 6ZSB; EM; 4.50 A; 1=1-65.
DR PDB; 6ZSC; EM; 3.50 A; 1=1-65.
DR PDB; 6ZSD; EM; 3.70 A; 1=1-65.
DR PDB; 6ZSE; EM; 5.00 A; 1=1-65.
DR PDB; 6ZSG; EM; 4.00 A; 1=1-65.
DR PDB; 7A5F; EM; 4.40 A; 13=1-65.
DR PDB; 7A5G; EM; 4.33 A; 13=1-65.
DR PDB; 7A5H; EM; 3.30 A; 1=1-65.
DR PDB; 7A5I; EM; 3.70 A; 13=1-65.
DR PDB; 7A5J; EM; 3.10 A; 1=1-65.
DR PDB; 7A5K; EM; 3.70 A; 13=1-65.
DR PDB; 7L08; EM; 3.49 A; 1=1-65.
DR PDB; 7L20; EM; 3.15 A; 1=1-65.
DR PDB; 7O9K; EM; 3.10 A; 1=1-65.
DR PDB; 7O9M; EM; 2.50 A; 1=1-65.
DR PDB; 7ODR; EM; 2.90 A; 1=1-65.
DR PDB; 7ODS; EM; 3.10 A; 1=1-65.
DR PDB; 7ODT; EM; 3.10 A; 1=1-65.
DR PDB; 7OF0; EM; 2.20 A; 1=1-65.
DR PDB; 7OF2; EM; 2.70 A; 1=1-65.
DR PDB; 7OF3; EM; 2.70 A; 1=1-65.
DR PDB; 7OF4; EM; 2.70 A; 1=1-65.
DR PDB; 7OF5; EM; 2.90 A; 1=1-65.
DR PDB; 7OF6; EM; 2.60 A; 1=1-65.
DR PDB; 7OF7; EM; 2.50 A; 1=1-65.
DR PDB; 7OG4; EM; 3.80 A; 1=1-65.
DR PDB; 7OI7; EM; 3.50 A; 1=1-65.
DR PDB; 7OI8; EM; 3.50 A; 1=1-65.
DR PDB; 7OI9; EM; 3.30 A; 1=1-65.
DR PDB; 7OIA; EM; 3.20 A; 1=1-65.
DR PDB; 7OIB; EM; 3.30 A; 1=1-65.
DR PDB; 7OIC; EM; 3.10 A; 1=1-65.
DR PDB; 7OID; EM; 3.70 A; 1=1-65.
DR PDB; 7OIE; EM; 3.50 A; 1=1-65.
DR PDB; 7PD3; EM; 3.40 A; 1=1-65.
DR PDB; 7QH6; EM; 3.08 A; 1=1-65.
DR PDBsum; 3IY9; -.
DR PDBsum; 3J7Y; -.
DR PDBsum; 3J9M; -.
DR PDBsum; 5OOL; -.
DR PDBsum; 5OOM; -.
DR PDBsum; 6I9R; -.
DR PDBsum; 6NU2; -.
DR PDBsum; 6NU3; -.
DR PDBsum; 6VLZ; -.
DR PDBsum; 6VMI; -.
DR PDBsum; 6ZM5; -.
DR PDBsum; 6ZM6; -.
DR PDBsum; 6ZS9; -.
DR PDBsum; 6ZSA; -.
DR PDBsum; 6ZSB; -.
DR PDBsum; 6ZSC; -.
DR PDBsum; 6ZSD; -.
DR PDBsum; 6ZSE; -.
DR PDBsum; 6ZSG; -.
DR PDBsum; 7A5F; -.
DR PDBsum; 7A5G; -.
DR PDBsum; 7A5H; -.
DR PDBsum; 7A5I; -.
DR PDBsum; 7A5J; -.
DR PDBsum; 7A5K; -.
DR PDBsum; 7L08; -.
DR PDBsum; 7L20; -.
DR PDBsum; 7O9K; -.
DR PDBsum; 7O9M; -.
DR PDBsum; 7ODR; -.
DR PDBsum; 7ODS; -.
DR PDBsum; 7ODT; -.
DR PDBsum; 7OF0; -.
DR PDBsum; 7OF2; -.
DR PDBsum; 7OF3; -.
DR PDBsum; 7OF4; -.
DR PDBsum; 7OF5; -.
DR PDBsum; 7OF6; -.
DR PDBsum; 7OF7; -.
DR PDBsum; 7OG4; -.
DR PDBsum; 7OI7; -.
DR PDBsum; 7OI8; -.
DR PDBsum; 7OI9; -.
DR PDBsum; 7OIA; -.
DR PDBsum; 7OIB; -.
DR PDBsum; 7OIC; -.
DR PDBsum; 7OID; -.
DR PDBsum; 7OIE; -.
DR PDBsum; 7PD3; -.
DR PDBsum; 7QH6; -.
DR AlphaFoldDB; O75394; -.
DR SMR; O75394; -.
DR BioGRID; 114925; 52.
DR ComplexPortal; CPX-5226; 39S mitochondrial large ribosomal subunit.
DR CORUM; O75394; -.
DR IntAct; O75394; 20.
DR STRING; 9606.ENSP00000296102; -.
DR PhosphoSitePlus; O75394; -.
DR BioMuta; MRPL33; -.
DR EPD; O75394; -.
DR jPOST; O75394; -.
DR MassIVE; O75394; -.
DR MaxQB; O75394; -.
DR PaxDb; O75394; -.
DR PeptideAtlas; O75394; -.
DR PRIDE; O75394; -.
DR ProteomicsDB; 49965; -. [O75394-1]
DR ProteomicsDB; 49966; -. [O75394-2]
DR TopDownProteomics; O75394-1; -. [O75394-1]
DR Antibodypedia; 58354; 113 antibodies from 23 providers.
DR DNASU; 9553; -.
DR Ensembl; ENST00000296102.8; ENSP00000296102.3; ENSG00000243147.8. [O75394-1]
DR Ensembl; ENST00000379666.7; ENSP00000368988.3; ENSG00000243147.8. [O75394-2]
DR GeneID; 9553; -.
DR KEGG; hsa:9553; -.
DR MANE-Select; ENST00000296102.8; ENSP00000296102.3; NM_004891.4; NP_004882.1.
DR UCSC; uc002rlm.1; human. [O75394-1]
DR CTD; 9553; -.
DR DisGeNET; 9553; -.
DR GeneCards; MRPL33; -.
DR HGNC; HGNC:14487; MRPL33.
DR HPA; ENSG00000243147; Low tissue specificity.
DR MIM; 610059; gene.
DR neXtProt; NX_O75394; -.
DR OpenTargets; ENSG00000243147; -.
DR PharmGKB; PA30964; -.
DR VEuPathDB; HostDB:ENSG00000243147; -.
DR eggNOG; KOG3505; Eukaryota.
DR GeneTree; ENSGT00390000010130; -.
DR HOGENOM; CLU_3124455_0_0_1; -.
DR InParanoid; O75394; -.
DR OMA; KHDPLVN; -.
DR OrthoDB; 1634766at2759; -.
DR PhylomeDB; O75394; -.
DR TreeFam; TF300279; -.
DR PathwayCommons; O75394; -.
DR Reactome; R-HSA-5368286; Mitochondrial translation initiation.
DR Reactome; R-HSA-5389840; Mitochondrial translation elongation.
DR Reactome; R-HSA-5419276; Mitochondrial translation termination.
DR SignaLink; O75394; -.
DR SIGNOR; O75394; -.
DR BioGRID-ORCS; 9553; 434 hits in 1094 CRISPR screens.
DR ChiTaRS; MRPL33; human.
DR GeneWiki; MRPL33; -.
DR GenomeRNAi; 9553; -.
DR Pharos; O75394; Tdark.
DR PRO; PR:O75394; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; O75394; protein.
DR Bgee; ENSG00000243147; Expressed in right adrenal gland cortex and 207 other tissues.
DR ExpressionAtlas; O75394; baseline and differential.
DR Genevisible; O75394; HS.
DR GO; GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
DR GO; GO:0005762; C:mitochondrial large ribosomal subunit; IDA:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0032543; P:mitochondrial translation; IC:ComplexPortal.
DR Gene3D; 2.20.28.120; -; 1.
DR InterPro; IPR038584; L33_sf.
DR InterPro; IPR001705; Ribosomal_L33.
DR InterPro; IPR011332; Ribosomal_zn-bd.
DR Pfam; PF00471; Ribosomal_L33; 1.
DR SUPFAM; SSF57829; SSF57829; 1.
DR TIGRFAMs; TIGR01023; rpmG_bact; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Mitochondrion; Reference proteome;
KW Ribonucleoprotein; Ribosomal protein; Transit peptide.
FT TRANSIT 1..8
FT /note="Mitochondrion"
FT /evidence="ECO:0000250|UniProtKB:Q3SZ47"
FT CHAIN 9..65
FT /note="39S ribosomal protein L33, mitochondrial"
FT /id="PRO_0000238625"
FT VAR_SEQ 14..65
FT /note="KNILVRMVSEAGTGFCFNTKRNRLREKLTLLHYDPVVKQRVLFVEKKKIRSL
FT -> NETKSPLRGKEKNTLPLNGGLKMTLIYKEKTEGGDTDSEIL (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_042822"
FT STRAND 15..22
FT /evidence="ECO:0007829|PDB:7OF0"
FT STRAND 29..38
FT /evidence="ECO:0007829|PDB:7OF0"
FT STRAND 42..47
FT /evidence="ECO:0007829|PDB:7OF0"
FT TURN 48..51
FT /evidence="ECO:0007829|PDB:7OF0"
FT STRAND 52..61
FT /evidence="ECO:0007829|PDB:7OF0"
SQ SEQUENCE 65 AA; 7619 MW; F32C16A238A5D08A CRC64;
MFLSAVFFAK SKSKNILVRM VSEAGTGFCF NTKRNRLREK LTLLHYDPVV KQRVLFVEKK
KIRSL
